二氢杨梅素-Ag+纳米乳液的制备及其对金黄色葡萄球菌抑制性能与机理研究

食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 基础研究 • 下一篇

二氢杨梅素-Ag+纳米乳液的制备及其对金黄色葡萄球菌抑制性能与机理研究

丁利君¹,黄梓浩²,刘丹¹

1. 广东工业大学轻工化工学院
2. 广东工业大学

收稿日期:2019-07-25 修回日期:2020-05-15 出版日期:2020-08-15 发布日期:2020-08-19
通讯作者: 黄梓浩 E-mail:1053385615@qq.com
基金资助:
脉冲电场介导碳纳米管灭活副溶血性弧菌机理研究

Preparation of Dihydromyricetin-Ag+ Nanoemulsion and Its Inhibition Performance and Mechanism on Staphylococcus aureus

¹,Zi-Hao HUANG

Received:2019-07-25 Revised:2020-05-15 Online:2020-08-15 Published:2020-08-19
Contact: Zi-Hao HUANG E-mail:1053385615@qq.com

摘要/Abstract

摘要： 摘要：对二氢杨梅素抑菌性能的初步探究，结果：二氢杨梅素对金黄色葡萄球菌的最低抑菌浓度（MIC）为1.25mg/mL。制备二氢杨梅素-银离子（DMY-Ag+）纳米乳液并表征，确认其水包油的结构（O/W）并可以稳定于水溶液中，通过全自动生长曲线、抑菌圈及平板计数的方法研究乳液对金黄色葡萄球菌的抑菌性能，发现其相比于DMY或Ag+都达到非常明显的协同效果，进一步探索乳液的抑菌机理，认为其一方面通过破坏细胞壁膜的完整性和选择透过性，使得胞内大小分子物质流向细胞外，另一方面造成细胞呼吸代谢循环链中的脱氢酶活下降或丧失，从而影响细胞的正常供能和生命活动，最终达到抑制或杀死细菌的目的。这对于将乳液作为一种天然高效的抑菌剂应用于食品行业提供了理论基础，具有很高的开发价值。

关键词: 关键词：二氢杨梅素、Ag+、纳米乳液、金黄色葡萄球菌、抑菌机理

Abstract: Abstract：A preliminary study on the antibacterial properties of dihydromyricetin showed that the minimum inhibitory concentration (MIC) of dihydromyricetin against Staphylococcus aureus was 1.25 mg/mL. The dihydromyricetin-silver ion (DMY-Ag+) nanoemulsion was prepared and characterized, and the structure of oil-in-water (O/W) was confirmed and stabilized in aqueous solution, and it was counted by automatic growth curve, inhibition zone and plate count. Methods The antibacterial activity of emulsion on Staphylococcus aureus was studied. It was found that it had a very obvious synergistic effect compared with DMY or Ag+. The antibacterial mechanism of emulsion was further explored, which was considered to destroy the integrity and selection of cell wall membrane on the one hand. Permeability, the intracellular large and small molecular substances flow to the outside of the cell, on the other hand, the dehydrogenase activity in the cellular respiratory metabolic chain is decreased or lost, thereby affecting the normal energy supply and life activities of the cells, and finally inhibiting or killing The purpose of the bacteria. This provides a theoretical basis for the application of emulsion as a natural and highly effective bacteriostatic agent in the food industry, and has a high development value.

Key words: Key words：dihydromyricetin,Ag+,nanoemulsion,Staphylococcus aureus,bacteriostatic mechanism

中图分类号:

TS201.3、TS201.6

丁利君黄梓浩刘丹. 二氢杨梅素-Ag+纳米乳液的制备及其对金黄色葡萄球菌抑制性能与机理研究[J]. 食品科学, 0, (): 0-0.

Zi-Hao HUANG. Preparation of Dihydromyricetin-Ag+ Nanoemulsion and Its Inhibition Performance and Mechanism on Staphylococcus aureus[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

参考文献
[1]马冉,雷柳冰,田国梁,李发俊.东莞市区肉菜市场熟肉制品中金黄色葡萄球菌污染溯源分析[J].食品安全质量检测学报,2019,10(12):3873-3877.
[2]郦娟,杨永,董华夏,涂凤琴.鲜湿米粉中金黄色葡萄球菌污染状况研究[J].粮食与油脂,2019,32(06):75-77.
[3]高璇.市售鲜牛奶中金黄色葡萄球菌的分离鉴定及耐药性分析[J].安徽农业科学,2018,46(27):173-175.
[4]黄郁梅,洪正善,杨柯,曾春晖.二氢杨梅素稳定性影响因素及抑菌活性研究进展[J].药学研究,2017,36(09):531-533+537.
[5]彭宏熙,李聪.中国和美国、日本饮用水水质标准的比较探究[J].中国给水排水,2018,34(10):26-31.
[6]马骉,李梦洁,陈志平.国内外饮用水标准比较及对我国未来水质标准的思考[J].中国给水排水,2016,32(10):11-14.
[7]陈日荣,李惠冰,周美容.不同种类微生物在全自动血培养仪中的生长曲线及其意义[J].中国感染控制杂志,2006(03):206-208+213.
[8]Barnard Ross T. The Zone of Inhibition.[J]. Clinical chemistry,2019,65(6).
[9]陈美莲,章慧,王仕英.Soleris微生物实时光电法与平板计数法测定生乳中菌落总数的比较研究[J].乳业科学与技术,2018,41(05):24-27.
[10]顾颖娟,秦宇,潘思奕,林毅侃,庞贝妮,刘洋.高效液相色谱法测定婴幼儿奶粉中5种核苷酸的含量[J].食品工业,2017,38(09):271-275.
[11]路阳,王贤舜,丁丽俐,王培之.用考玛斯亮兰G—250迅速、灵敏地测定蛋白质浓度[J].生物学杂志,1992(01):24-25+32.
[12]管斌,丁友昉,谢来苏,隆言泉.还原糖测定方法的规范[J].无锡轻工大学学报,1999(03):74-79.
[13]郑志永,姚善泾.比色法测定大肠杆菌呼吸链脱氢酶活性[J].浙江大学学报(工学版),2005(03):119-122.
[14]Dan Liu,Wencong Pang,Lijun Ding,Jianxia Sun. An insight into the inhibitory activity of dihydromyricetin against Vibrio parahaemolyticus[J]. Food Control,2016,67.
[15]肖小年,王江南,谭潇啸,胡居吾,易醒.二氢杨梅素的抑菌活性及其影响因素[J].中国食品学报,2016,16(10):124-129.
[16]熊伟,李雄辉,王慧宾,袁菊如,王金昌.二氢杨梅素的抑菌性能研究及其与苯甲酸钠的比较[J].生物化工,2016,2(01):12-14.
[17]王江南. 二氢杨梅素的抑菌作用研究[D].南昌大学,2014.
[18]Fuad Ameen,Sami A. AlYahya,Muhammed A. Bakhrebah,Majed S. Nassar,Amal Aljuraifani. Flavonoid dihydromyricetin-mediated silver nanoparticles as potential nanomedicine for biomedical treatment of infections caused by opportunistic fungal pathogens[J]. Research on Chemical Intermediates,2018,44(9).
[19]熊伟,王慧宾,谭潇啸,肖小年,李雄辉.二氢杨梅素对金黄色葡萄球菌细胞渗透性的影响研究[J].食品工业,2016,37(01):214-216.
[20]熊伟,王慧宾,谭潇啸,肖小年,李雄辉.二氢杨梅素对细菌形态及呼吸代谢的影响研究[J].食品工业,2015,36(11):12-14.
[21]Hengyi Xu,Feng Qu,Hong Xu,Weihua Lai,Y. Andrew Wang,Zoraida P. Aguilar,Hua Wei. Role of reactive oxygen species in the antibacterial mechanism of silver nanoparticles on Escherichia coli O157:H7[J]. BioMetals,2012,25(1).
[22]Wen-Ru Li,Xiao-Bao Xie,Qing-Shan Shi,Hai-Yan Zeng,You-Sheng OU-Yang,Yi-Ben Chen. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli[J]. Applied Microbiology and Biotechnology,2010,85(4).参考文献
[1]马冉,雷柳冰,田国梁,李发俊.东莞市区肉菜市场熟肉制品中金黄色葡萄球菌污染溯源分析[J].食品安全质量检测学报,2019,10(12):3873-3877.
[2]郦娟,杨永,董华夏,涂凤琴.鲜湿米粉中金黄色葡萄球菌污染状况研究[J].粮食与油脂,2019,32(06):75-77.
[3]高璇.市售鲜牛奶中金黄色葡萄球菌的分离鉴定及耐药性分析[J].安徽农业科学,2018,46(27):173-175.
[4]黄郁梅,洪正善,杨柯,曾春晖.二氢杨梅素稳定性影响因素及抑菌活性研究进展[J].药学研究,2017,36(09):531-533+537.
[5]彭宏熙,李聪.中国和美国、日本饮用水水质标准的比较探究[J].中国给水排水,2018,34(10):26-31.
[6]马骉,李梦洁,陈志平.国内外饮用水标准比较及对我国未来水质标准的思考[J].中国给水排水,2016,32(10):11-14.
[7]陈日荣,李惠冰,周美容.不同种类微生物在全自动血培养仪中的生长曲线及其意义[J].中国感染控制杂志,2006(03):206-208+213.
[8]Barnard Ross T. The Zone of Inhibition.[J]. Clinical chemistry,2019,65(6).
[9]陈美莲,章慧,王仕英.Soleris微生物实时光电法与平板计数法测定生乳中菌落总数的比较研究[J].乳业科学与技术,2018,41(05):24-27.
[10]顾颖娟,秦宇,潘思奕,林毅侃,庞贝妮,刘洋.高效液相色谱法测定婴幼儿奶粉中5种核苷酸的含量[J].食品工业,2017,38(09):271-275.
[11]路阳,王贤舜,丁丽俐,王培之.用考玛斯亮兰G—250迅速、灵敏地测定蛋白质浓度[J].生物学杂志,1992(01):24-25+32.
[12]管斌,丁友昉,谢来苏,隆言泉.还原糖测定方法的规范[J].无锡轻工大学学报,1999(03):74-79.
[13]郑志永,姚善泾.比色法测定大肠杆菌呼吸链脱氢酶活性[J].浙江大学学报(工学版),2005(03):119-122.
[14]Dan Liu,Wencong Pang,Lijun Ding,Jianxia Sun. An insight into the inhibitory activity of dihydromyricetin against Vibrio parahaemolyticus[J]. Food Control,2016,67.
[15]肖小年,王江南,谭潇啸,胡居吾,易醒.二氢杨梅素的抑菌活性及其影响因素[J].中国食品学报,2016,16(10):124-129.
[16]熊伟,李雄辉,王慧宾,袁菊如,王金昌.二氢杨梅素的抑菌性能研究及其与苯甲酸钠的比较[J].生物化工,2016,2(01):12-14.
[17]王江南. 二氢杨梅素的抑菌作用研究[D].南昌大学,2014.
[18]Fuad Ameen,Sami A. AlYahya,Muhammed A. Bakhrebah,Majed S. Nassar,Amal Aljuraifani. Flavonoid dihydromyricetin-mediated silver nanoparticles as potential nanomedicine for biomedical treatment of infections caused by opportunistic fungal pathogens[J]. Research on Chemical Intermediates,2018,44(9).
[19]熊伟,王慧宾,谭潇啸,肖小年,李雄辉.二氢杨梅素对金黄色葡萄球菌细胞渗透性的影响研究[J].食品工业,2016,37(01):214-216.
[20]熊伟,王慧宾,谭潇啸,肖小年,李雄辉.二氢杨梅素对细菌形态及呼吸代谢的影响研究[J].食品工业,2015,36(11):12-14.
[21]Hengyi Xu,Feng Qu,Hong Xu,Weihua Lai,Y. Andrew Wang,Zoraida P. Aguilar,Hua Wei. Role of reactive oxygen species in the antibacterial mechanism of silver nanoparticles on Escherichia coli O157:H7[J]. BioMetals,2012,25(1).
[22]Wen-Ru Li,Xiao-Bao Xie,Qing-Shan Shi,Hai-Yan Zeng,You-Sheng OU-Yang,Yi-Ben Chen. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli[J]. Applied Microbiology and Biotechnology,2010,85(4).

二氢杨梅素-Ag+纳米乳液的制备及其对金黄色葡萄球菌抑制性能与机理研究

Preparation of Dihydromyricetin-Ag+ Nanoemulsion and Its Inhibition Performance and Mechanism on Staphylococcus aureus

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价