食品科学 ›› 2019, Vol. 40 ›› Issue (20): 178-184.doi: 10.7506/spkx1002-6630-20180819-190

• 生物工程 • 上一篇    下一篇

基于高通量分析流通方式对大目金枪鱼品质与微生物种群变化影响

蓝蔚青,车旭,巩涛硕,张墨言,孙晓红,肖蕾,谢晶   

  1. (1.上海海洋大学食品学院,上海 201306;2.上海水产品加工及贮藏工程技术研究中心,上海冷链装备性能与节能评价专业技术服务平台,食品科学与工程国家级实验教学示范中心(上海海洋大学),上海 201306)
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    现代农业产业技术体系建设专项(CARS-47);上海水产品加工及贮藏工程技术研究中心能力提升项目(19DZ2284000)

LAN Weiqing, CHE Xu, GONG Taoshuo, ZHANG Moyan, SUN Xiaohong, XIAO Lei, XIE Jing

LAN Weiqing, CHE Xu, GONG Taoshuo, ZHANG Moyan, SUN Xiaohong, XIAO Lei, XIE Jing   

  1. (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China)
  • Online:2019-10-25 Published:2019-10-25

摘要: 为综合分析大目金枪鱼在冷链(CK)与断链(L1)流通过程中的品质与菌群结构变化,以期为水产品流通保鲜提供理论参考,分别对两种流通方式的温度波动进行模拟,通过理化指标(pH值与总挥发性盐基氮(total volatile base nitrogen,TVB-N)值)、微生物指标(菌落总数与嗜冷菌数)表征其品质变化,由高通量测序研究两种流通方式在各流通阶段的微生物菌群变化规律。结果表明,CK组样品在-55 ℃的各指标几乎不发生变化,4 ℃后样品中的微生物指标明显增长。L1组样品随着温度波动,其pH值呈先降后升的变化趋势,TVB-N值、菌落总数与嗜冷菌数均高于CK组样品,且在168 h后由于温度波动,其变化趋势显著,样品超出生食标准。高通量测序结果发现,CK组样品在流通末期(SM-HR)的优势菌主要为热死环丝菌(Brochothrix thermosphacta)、内生菌(Endophytes)、雷尔氏菌(Ralstonia solanacearum)、不动杆菌属(Acinetobacter spp.)、芽孢杆菌属(Bacillus spp.)与假单胞菌属(Pseudomonas spp.);而L1组样品在流通末期(R-FT)的主要菌为热死环丝菌、内生菌、雷尔氏菌、不动杆菌属、溶杆菌(Lysobacter)、假交替单胞菌属(Pseudoalteromonas spp.)、嗜冷杆菌属(Psychrobacter spp.)与希瓦氏菌属(Shewanella spp.)。

关键词: 大目金枪鱼, 高通量测序, 流通过程, 品质, 微生物种群

Abstract: The changes in the quality and microbial population structure of big-eye tuna during cold chain (CK) or logistic process (L1) were analyzed comprehensively, aiming to provide a theoretical basis for the circulation and preservation of aquatic products. The temperature fluctuations under the CK and L1 modes were simulated in this paper. Physicochemical parameters including pH and total volatile base nitrogen (TVB-N) and microbial parameters like total viable count (TVC) and Psychrobacter count (PC) were measured during the two processes, and the microbial diversity was studied by high-throughput sequencing analysis. The results showed that the quality and microbial diversity of the CK group at ?55 ℃ were stable, but the microbial indexes increased considerably at 4 ℃. The pH value of the L1 group decreased first and then increased. TVB-N, TVC and PC of L1 were higher than those of CK, which exceeded the safety limit because of temperature fluctuations after 168 h. The results of high-throughput sequencing showed that the dominant bacteria in the CK group at the end of circulation (SM-HR) were mainly Brochothrix thermosphacta, Endophytes, Ralstonia solanacearum, Acinetobacter spp., Bacillus spp. and Pseudomonas spp., and the main bacteria in the L1 group at the end of circulation (R-FT) were B. thermosphacta, Endophytes, R. solanacearum, Acinetobacter spp., Lysobacter, Pseudoalteromonas spp., Psychrobacter spp. and Shewanella spp..

Key words: Thunnus obesus, high-throughput sequencing, circulation, quality, microbial population

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