食品科学 ›› 2019, Vol. 40 ›› Issue (20): 279-285.doi: 10.7506/spkx1002-6630-20181018-197

• 工艺技术 • 上一篇    下一篇

超声波辅助法制备牛骨源柠檬酸钙的工艺优化

单媛媛,刘叶虹萱,代雨柔,周元,伊扬磊,王欣,刘变芳,吕欣   

  1. (西北农林科技大学食品科学与工程学院,陕西 杨凌 712100)
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    陕西省重点研发计划一般项目(2017NY-150)

SHAN Yuanyuan, LIU Yehongxuan, DAI Yurou, ZHOU Yuan, YI Yanglei, WANG Xin, LIU Bianfang, Lü Xin

SHAN Yuanyuan, LIU Yehongxuan, DAI Yurou, ZHOU Yuan, YI Yanglei, WANG Xin, LIU Bianfang, Lü Xin   

  1. (College of Food Science & Engineering, Northwest A&F University, Yangling 712100, China)
  • Online:2019-10-25 Published:2019-10-25

摘要: 以废弃黄牛长骨为原料,在单因素试验基础上以柠檬酸钙转化率为指标,通过响应面试验研究超声辅助直接中和法制备牛骨源柠檬酸钙的工艺,并与常规方法制备牛骨柠檬酸钙的效果进行比较分析。结果表明,超声辅助制备牛骨源柠檬酸钙的最佳工艺条件为:料液比1∶15(g/mL)、料酸比1∶2、超声功率320 W、超声温度39 ℃、超声时间21 min。在此条件下柠檬酸钙的转化率为71.21%,所得柠檬酸钙的纯度为94.38%。对超声辅助直接中和法与传统溶钙技术的离子钙转化率进行比较,发现超声辅助直接中和法反应21 min后离子钙含量比静置法、搅拌法、蒸煮法反应60 min后的离子钙含量分别提高了84、4.5 倍和3 倍。超声波处理3 次后牛骨钙的溶出率高达96.18%。结果说明超声波辅助法是将牛骨中钙离子化的有效方法,可为以牛骨为原料工业生产钙制品提供参考。

关键词: 牛长骨, 柠檬酸钙, 超声波, 响应面优化

Abstract: In order to make full use of calcium resources in bovine bone, bovine bone waste was chosen as starting material to prepare calcium citrate by an ultrasonic-assisted direct neutralization method and the process conditions were optimized by the combined use of one-factor-at-a-time method and response surface methodology based on the yield of calcium citrate. The new preparation method was evaluated in comparison to the routine calcium dissolution method. Results showed that the optimum process conditions were determined as follows: solid-to-solvent ratio 1:15 (g/mL), solid-to-citric acid ratio 1:2, ultrasonic power 320 W, temperature 39 ℃, and sonication time 21 min, giving a calcium citrate yield of 71.21%. The purity of the product prepared using the optimized conditions was 94.38%. The amount of dissolved calcium after 21 min reaction in the new method was 85, 5.5 and 4 times as high as that obtained by 60 min standing, stirring or cooking without ultrasonic treatment, respectively. The amount of dissolved calcium was as high as 96.18% after three cycles of ultrasonic treatment. These results confirmed the ultrasonic-assisted method to be an effective method for preparing dissolved organic calcium from bovine bone, which can provide a basis for the industrial production of calcium products from waste bovine bone.

Key words: bovine bone, calcium citrate, ultrasonic, response surface methodology

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