食品科学 ›› 2019, Vol. 40 ›› Issue (20): 332-337.doi: 10.7506/spkx1002-6630-20180827-295

• 安全检测 • 上一篇    下一篇

固相萃取-超高效液相色谱-串联质谱法测定饮用水中双酚A和双酚S

杨金泉,贺小敏,施敏芳,陈浩,李永蓉,朱友林   

  1. (1.湖北省环境监测中心站,湖北 武汉 430072;2.华中农业大学理学院,湖北 武汉 430070;3.长江大学资源与环境学院,湖北 武汉 430100)
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    湖北省环境保护厅环保科研项目(2017HB02)

YANG Jinquan, HE Xiaomin, SHI Minfang, CHEN Hao, LI Yongrong, ZHU Youlin

YANG Jinquan, HE Xiaomin, SHI Minfang, CHEN Hao, LI Yongrong, ZHU Youlin   

  1. (1. Hubei Provincial Environmental Monitoring Center Station, Wuhan 430072, China;2. College of Science, Huazhong Agricultural University, Wuhan 430070, China; 3. College of Resources and Environment, Yangtze University, Wuhan 430100, China)
  • Online:2019-10-25 Published:2019-10-25

摘要: 建立一种饮用水中痕量双酚A和双酚S的固相萃取-超高效液相色谱-串联质谱检测方法。水样中双酚A和双酚S经HLB固相萃取柱富集,甲醇洗脱并浓缩,再以1 mmol/L氟化铵-乙腈为流动相,经C18色谱柱分离,采用电喷雾离子源、质谱多反应监测模式分析。该方法对水样体积为500 mL时双酚A和双酚S检出限分别可达到1.0 ng/L和0.2 ng/L,在低、中、高3 个加标水平下两者的平均回收率分别为88.3%~114.8%和83.7%~98.2%,相对标准偏差分别为10.0%~16.2%和5.9%~12.3%。该方法灵敏度高、定性准确、操作简单高效,适用于饮用水中双酚A和双酚S的定性定量分析。

关键词: 双酚A, 双酚S, 液相色谱-串联质谱法, 饮用水, 固相萃取

Abstract: An analytical method was established for the determination of bisphenol A (BPA) and bisphenol S (BPS) in drinking water by solid-phase extraction and ultra performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS). BPA and BPS from samples were enriched by an Oasis HLB SPE cartridge, eluted with methanol and concentrated with nitrogen. The analytes were separated on an C18 column with 1 mmol/L NH4F aqueous-acetonitrile as the mobile phase, and then analyzed by UPLC-MS/MS using multiple reaction monitoring (MRM) under the negative-ion mode. The limits of detection (LODs) of the method for BPA and BPS were 1.0 and 0.2 ng/L, respectively. The mean recoveries at three spiked levels of BPA ranged from 88.3% to 114.8%, with relative standard deviations (RSDs) of 10.0%–16.2%. The recoveries of BPS were 83.7%–98.2%, with RSDs of 5.9%–12.3%. The method was proved to be sensitive, accurate and efficient, and it was suitable for qualitative and quantitative analysis of BPA and BPS in drinking water.

Key words: bisphenol A, bisphenol S, ultra performance liquid chromatography-tandem mass spectrometry, drinking water, solid-phase extraction

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