关键词: 荷叶, 超高效液相色谱-四极杆飞行时间串联质谱, 化学成分, 鉴定

Abstract: This research was designed to identify the major chemical constituents of the ethyl acetate fraction of lotus leaf extract by using ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). The compounds were identified based on the accurate molecular mass, parent ions, MS/MS fragment ions, and MS/MS fragmentation pattern of each peak, along with the information about the compounds previously identified in lotus leaves as well as available literature data. Totally, 40 compounds were identified, including 27 flavonoids, 6 phenolic acids, 4 organic acids, 2 fatty acids and 1 amino acid. Flavonoids were the major chemical constituents with quercetin glycosides, kaempferol glycosides, isorhamnetin glycosides and flavan-3-ols being the predominant ones, followed by phenolic acids. This research can provide a theoretical foundation for the application of lotus leaves in the food processing industry and for health-promoting food development.

Key words: lotus leaf, ultra-high performance liquid chromatography-quadrupole time-of-light tandem mass spectrometry, chemical constituents, identification