食品科学 ›› 2019, Vol. 40 ›› Issue (24): 40-45.doi: 10.7506/spkx1002-6630-20181114-164

• 食品化学 • 上一篇    下一篇

松仁清蛋白抗氧化肽的分离纯化及结构鉴定

卢红妍,杨行,方丽,刘诗梦,闵伟红   

  1. (1.吉林农业大学食品科学与工程学院,吉林 长春 130118;2.小麦和玉米深加工国家工程实验室,吉林 长春 130118)
  • 出版日期:2019-12-25 发布日期:2019-12-24
  • 基金资助:
    国家高技术研究发展计划(863计划)项目(2013AA102206);吉林省教育厅“十三五”科学技术项目(JJKH20170300KJ)

Isolation, Purification and Structural Identification of Antioxidant Peptides Derived from Enzymatic Hydrolysis of Pine Nut Kernel (Pinus koraiensis Sieb. et Zucc.) Albumin

LU Hongyan, YANG Hang, FANG Li, LIU Shimeng, MIN Weihong   

  1. (1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;2. National Engineering Laboratory on Wheat and Corn Further Processing, Changchun 130118, China)
  • Online:2019-12-25 Published:2019-12-24

摘要: 利用碱性蛋白酶酶解松仁清蛋白制备高抗氧化活性肽,通过超滤、SephadexG-25、SephadexG-15及反相高效液相色谱对抗氧化肽进行分离纯化,并采用电喷雾串联质谱进行结构鉴定。经筛选获得高抗氧化活性肽苯丙氨酸-苯丙氨酸-脯氨酸-酪氨酸(FFPY)和酪氨酸-亮氨酸-脯氨酸-苯丙氨酸(YLPF),分子质量分别为572.67 Da和538.65 Da。对FFPY和YLPF进行固相合成,纯度达到99.16%和99.91%,氧自由基吸收能力分别为7 329.63 μmol/g和2 835.47 μmol/g。

关键词: 松仁清蛋白, 抗氧化肽, 分离纯化, 结构鉴定

Abstract: Two peptides with strong antioxidant activity were seprate and purified from the alkaline protease of pine nut kernel albumin hydrolysate by by ultrafiltration, column chromatographies on Sephadex G-25, and Sephadex G-15 and reversed-phase high-performance liquid chromatography, and their structure was identified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The peptides were found to be Phe-Phe-Pro-Tyr (FFPY) and Tyr-Leu-Pro-Phe (YLPF) with molecular masses were 572.67 and 538.65 Da, respectively. Solid phase synthesis of FFPY and YLPF was performed with a purity of 99.16% and 99.91%. It was proved that the oxygen radical absorbance capacity (ORAC) values of FFPY and YLPF were 7 329.63 and 2 835.47 μmol/g, respectively.

Key words: pine nut kernel albumin, antioxidant peptides, separation and purification, structural identification

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