食品科学 ›› 2020, Vol. 41 ›› Issue (1): 1-8.doi: 10.7506/spkx1002-6630-20181121-250

• 基础研究 •    下一篇

热压结合处理对绿色魏斯氏菌生理特性的影响

余龙霞,吴香,李新福,张万刚,李聪,徐宝才   

  1. (1.南京农业大学食品科学技术学院,江苏省肉类生产与加工质量安全控制协同创新中心,肉品加工与质量控制教育部重点实验室,江苏 南京 210095;2.江苏雨润肉食品有限公司,肉品加工与质量控制国家重点实验室,江苏 南京 211806;3.合肥工业大学食品与生物工程学院,安徽 合肥 230601)
  • 出版日期:2020-01-15 发布日期:2020-01-19
  • 基金资助:
    国家自然科学基金面上项目(31571909)

Effect of High Pressure Thermal Sterilization on Physiological Characteristics of Weissella viridescens

YU Longxia, WU Xiang, LI Xinfu, ZHANG Wangang, LI Cong, XU Baocai   

  1. (1. Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. State Key Laboratory of Meat Processing and Quality Control, Jiangsu Yurun Meat Foods Co. Ltd., Nanjing 211806, China; 3. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)
  • Online:2020-01-15 Published:2020-01-19

摘要: 为了探究热压结合处理在绿色魏斯氏菌致死过程中发挥的作用。本实验采用超高压(350?MPa、25?℃、10?min)和热压结合(350?MPa、50?℃、10?min)对绿色魏斯氏菌进行压力处理,研究了热压结合处理对绿色魏斯氏菌生理特性、细胞形态特征、细胞膜的通透性等的影响。结果显示,热压结合处理使菌落总数从超高压处理时的7.67(lg(CFU/mL))显著下降到5.71(lg(CFU/mL))(P<0.05),协同的热处理使菌体表面出现严重褶皱、菌体内含物变性凝固加剧,使细胞膜损伤,从而改变膜的通透性,导致细胞外紫外吸收物质显著增加(P<0.05)。因此,热压结合处理在超高压致死细菌中会加剧细胞膜的形变及损坏,增加细胞膜的通透性,从而导致细胞内紫外吸收物质的流出,实验结果为热压结合灭活绿色魏斯氏菌提供了理论依据。

关键词: 热压结合灭菌, 绿色魏斯氏菌, 细胞膜损伤

Abstract: To explore the killing effect of high pressure thermal sterilization (HPTS) on Weissella viridescens, the physiological properties, morphological characteristics and membrane permeability of bacterial cells treated by either ultra high pressure (UHP) (at 350 MPa and 25 ℃ for 10 min) or HPTS (at 350 MPa and 50 ℃ for 10 min) were evaluated. The results showed that after HPTS treatment total bacteria count was significantly (P < 0.05) lower than that after UHP treatment (5.71 versus 7.67 (lg(CFU/mL))). The synergistic treatment caused the surface of bacterial cells to become seriously wrinkled, caused denaturation and coagulation of cellular contents, and damaged the cell membrane thereby altering membrane permeability and resulting in a significant increase in extracellular ultraviolet (UV)-absorbing substances (P < 0.05). In summary, HPTS could aggravate deformation of bacterial cells and increase cell membrane permeability leading to leakage of intracellular UV-absorbing substances. The results of this study provide theoretical evidence for the mechanisms of Weissella viridescens inactivation by HPTS.

Key words: high pressure thermal sterilization, Weissella viridescens, cell membrane damage

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