产蛋白酶菌株的筛选及以菜籽粕为氮源的产酶条件优化

摘要/Abstract

摘要： 本研究从南昌大学食堂污水中筛选出一株高产蛋白酶菌株CL-10，通过生理生化试验以及16S rRNA测序鉴定为解淀粉芽孢杆菌。以菜籽粕为氮源，以蛋白酶酶活为指标，通过对培养基成分单因素试验探讨不同因素对发酵产蛋白酶的影响，在此基础上，通过响应面优化菜籽粕、玉米粉、麸皮的含量，结果表明，在菜籽粕含量6.1%、玉米粉含量4.4%、麸皮含量3.2%、吐温20浓度0.7%、ZnSO4·7H2O浓度0.2%的最优条件下，蛋白酶酶活的验证值为6385.1U/ml，相比基础发酵培养基酶活2715U/ml，提高了1.35倍。

关键词: 菌种筛选, 蛋白酶, 解淀粉芽孢杆菌, 菜籽粕, 响应面优化

Abstract: In this study, a high-protease strain named CL-10 was screened from the canteen sewage in Nanchang University, and was identified as Bacillus amyloliquefaciens through physiological and biochemical experiments and 16S rRNA sequencing technology. Using the protease activity of Bacillus amyloliquefaciens as the response value, a single factor experiment was applied to optimize the on the components of enzyme-producing culture medium whose nitrogen source was rapeseed meal. Besides, the contents of rapeseed meal, corn meal and bran were particularly optimized by response surface method. As a result, the optimal components were as follow: the rapeseed meal content of 6.1%, the corn flour content of 4.4%, the bran content of 3.2%, the Tween 20 concentration of 0.7%, and the ZnSO4 · 7H2O concentration of 0.2%. On this condition, the maximum activity was up to 6385.1U / ml, which was 1.35 times higher than that of 2715U / ml in the basic fermentation medium.

Key words: strain screening, protease, Bacillus amyloliquefaciens, rapeseed meal, response surface optimization

中图分类号:

Q815

曾哲灵. 产蛋白酶菌株的筛选及以菜籽粕为氮源的产酶条件优化[J]. 食品科学, 0, (): 0-0.

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