酪蛋白糖基化对其消化物在模型小鼠中免疫活性的影响

摘要/Abstract

摘要： 本研究揭示酪蛋白的美拉德糖基化反应对酪蛋白消化物在免疫低下小鼠中免疫活性的影响。酪蛋白与乳糖发生美拉德糖基化反应得到糖基化酪蛋白，利用胰蛋白酶消化分别制备酪蛋白消化物和糖基化酪蛋白消化物，并用80 mg/kg BW的环磷酰胺处理小鼠3天以诱导其免疫低下；100 mg/kg、200 mg/kg和400 mg/kg BW的消化物剂量灌胃小鼠25 天后，测定小鼠免疫器官重量指数（脾脏指数和胸腺指数）、血清生化指标、血清免疫球蛋白（IgM、IgA和IgG）含量、脾淋巴细胞增殖作用和NK细胞活性。结果发现，与生理盐水处理的正常组小鼠相比，模型组小鼠的免疫状况低下，其免疫器官重量指数、血清生化指标、血清中免疫球蛋白含量、脾淋巴细胞增殖和NK细胞活性等指标明显降低（P< 0.05）；然而，与模型组小鼠相比，中、高剂量酪蛋白消化物灌胃的小鼠，其脾脏指数和胸腺指数分别增加35.9%-66.4%和50.5%?62.9%，血清IgM、IgA和IgG分别增加21.6%-30.4%、27.1%-41.8%和37.2%-45.1%，脾淋巴细胞增殖及NK细胞活性分别增加34.2%-51.1%和32.1%-90.7%。中、高剂量糖基化酪蛋白消化物灌胃的小鼠，其脾脏指数和胸腺指数分别增加27.7%-51.9%和39.2%-52.6%，血清IgM、IgA和IgG含量分别增加19.1%-29.0%、21.8%-35.3%和30.1%-41.6%。脾淋巴细胞增殖及NK细胞活性分别增加32.9%-41.6%和27.3%-81.1%。酪蛋白消化物和糖基化酪蛋白消化物均可明显减轻由环磷酰胺引起的上述免疫指标下降，并且，酪蛋白消化物比糖基化酪蛋白消化物有更好的免疫指标提升作用。所以，酪蛋白的美拉德糖基化反应会降低糖基化酪蛋白消化物对小鼠免疫状况的提高作用。

关键词: 酪蛋白, 美拉德反应, 糖基化, 小鼠, 免疫作用

Abstract: This study aimed to assess the potential effect of lactose glycation of caseinate via the Maillard reaction on in vivo immuno-enhancing efficacy of the yielded tryptic caseinate digest. A lactose-glycated caseinate was prepared using the Maillard reaction, caseinate, and lactose. Both glycated caseinate and caseinate were digested with trypsin to generate respective glycated caseinate digest and caseinate digest. The mice were treated with cyclophosphamide of 80 mg/kg for 3 days to induce immuno-suppression, and then were given the two digests at dose levels of 100, 200, and 400 mg/kg BW for another 25 days. After that, the mice were measured for their immune states by assaying spleen and thymus indices, hematological parameters, immunoglobulin (IgM, IgA, and IgG) production, lymphocyte proliferation, and NK cell activity as evaluation indices. Results indicated that administration of cyclophosphamide decreased all evaluation indices of the suppressed mice, compared with the physiological saline-treated normal mice. However, compared with the suppressed mice, the mice treated with caseinate digest at medium- and high-doses had 35.9%-66.4% and 50.5%-62.9% increases in spleen and thymus indices, 21.6%-30.4%, 27.1%-41.8%, and 37.2%-45.1% increases in serum IgM, IgA, and IgG production, and 34.2%-51.1% and 32.1%-90.7% increases in lymphocyte proliferation and NK cell activity, respectively. The mice treated with glycated caseinate digest at medium- and high-doses showed 27.7%-51.9% and 39.2%-52.6% increases in spleen and thymus indices, 19.1%-29.0%, 21.8%-35.3% and 30.1%-41.6% increases in serum IgM, IgA, and IgG production, and 32.9%-41.6% and 27.3%-81.1% increases in lymphocyte proliferation and NK cell activity, respectively. Both two digests were able to alleviate index decreases of the mice caused by the cyclophosphamide, while caseinate digest always showed higher immuno-enhancing efficacy than glycated caseinate digest. It is thus concluded that prior caseinate glycation via the Maillard reaction might decrease the effect of caseinate digest to enhancing immune status of the mice.

Key words: Caseinate, Maillard reaction, glycation, mice, immune effect

中图分类号:

TS201.4

时佳刘宛宁付余赵新淮. 酪蛋白糖基化对其消化物在模型小鼠中免疫活性的影响[J]. 食品科学, 0, (): 0-0.

Xin-Huai Zhao. Effect of caseinate glycation on immuno-enhancing activities of tryptic caseinate digest in the immuno-suppressed mice[J]. FOOD SCIENCE, 0, (): 0-0.

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