Akkermansia muciniphila在肠道消化模拟系统中的变化规律

摘要/Abstract

摘要： Akkermansia muciniphila是潜在的下一代益生菌，但其在中国人群肠道中的定植情况尚不明确。本研究收集了广州地区131个人体粪便样本，分析A.muciniphia的定植率，再挑选无A.muciniphia定植的人群的粪便作为体外肠道消化模拟系统的接种物，添加1×109 CFU的A.muciniphia进行干预，通过荧光定量PCR和高通量测序评估A.muciniphia的生长情况和对体外肠道微生态的影响。结果显示：A.muciniphia在测试人群（n=131）的定植率为89.31%，平均丰度为5.825 lg (CFU/mL)；A.muciniphia进入肠道消化模拟系统后，在肠道微生态环境中维持生长状态，对Lactobacillus属有增殖作用，但抑制肠道中的Bifidobacteria属和Bacteroidetes属；测序结果进一步发现在菌落总体结构上，A.muciniphia干预后对肠道微生态的结构无显著影响。结论：A.muciniphia在新的肠道环境中具备生存能力，对人体肠道微生态没有破坏性。

关键词: A.muciniphila , 肠道消化模拟系统, 肠道微生态

Abstract: Among the next-generation beneficial microbes that have been identified, A.muciniphila is a promising candidate, but its colonization in the intestinal tracts of Chinese populations is not clear. We collected 131 human fecal samples from Guangzhou, and analyzed the colonization rate of A.muciniphila. Then, we chose the feces which was free of A.muciniphia as the inoculum and 1×109 CFU of A.muciniphia was spiked into an in-vitro model to evaluate the colonization ability of A.muciniphia by qPCR and high-throughput sequencing. The results of qPCR showed that the colonization rate was 89.31% and to average abundance was 5.825 LOG (cfu/mL). The abundance of A.muciniphia changed slightly in the first 24h period. The growth of Lactobacillus genus was stimulated and the growth of Bifidobacteria genus and Bacteroidetes genus were inhibited. The results of NGS showed that the microbial profiles before and after the interference of A.muciniphila had no significant different. In conclusion, A.muciniphila had certain ability to colonize, and it did not destruct the intestinal microbiota.

Key words: A.muciniphila, gastrointestinal simulation system, intestinal microecology

中图分类号:

Q93-33

王磊吴希阳姚泓汤新. Akkermansia muciniphila在肠道消化模拟系统中的变化规律[J]. 食品科学, 0, (): 0-0.

Lei /WANG Xiyang Wu. The changes of abundance of Akkermansia muciniphila in an in-vitro gut fermentation model[J]. FOOD SCIENCE, 0, (): 0-0.

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