食品科学 ›› 2020, Vol. 41 ›› Issue (5): 256-265.doi: 10.7506/spkx1002-6630-20190127-352

• 专题论述 • 上一篇    下一篇

γ-kokumi肽及其合成酶γ-谷氨酰转肽酶的研究进展

伍圆明,伍伦杰,王莉,林璐,刘义,孙伟峰,丁文武   

  1. (1.西华大学食品与生物工程学院,四川 成都 610039;2.衡阳师范学院生命科学与环境学院,湖南 衡阳 421008)
  • 出版日期:2020-03-15 发布日期:2020-03-23
  • 基金资助:
    国家自然科学基金青年科学基金项目(31601418);四川省科技厅应用基础项目(2019YJ0390); 衡阳师范学院人才引进项目(18D14);四川省教育厅项目(15ZB0126)

Recent Advances in γ-Kokumi Peptide and Its Synthetase γ-Glutamyl Transpeptidase

WU Yuanming, WU Lunjie, WANG Li, LIN Lu, LIU Yi, SUN Weifeng, DING Wenwu   

  1. (1. School of Food and Biotechnology, Xihua University, Chengdu 610039, China; 2. College of Life Science and Environment, Hengyang Normal University, Hengyang 421008, China)
  • Online:2020-03-15 Published:2020-03-23

摘要: Kokumi是第6种基本味觉候选之一,添加到食物中能增强味道的浓厚感、饱满感、持续性及协调性,其相关增味产品具有巨大的开发价值和市场前景。Kokumi物质中重要的一类是具有kokumi味的γ-谷氨酰肽,称为γ-kokumi肽,由γ-谷氨酰基及氨基酸/多肽两部分组成,可通过具有转肽活性的γ-谷氨酰转肽酶(γ-glutamyl transpeptidase,GGT)合成。因此,本文对γ-kokumi肽结构特征、食物来源、呈味评价方法、制备方法、GGT结构特征、催化机理及合成γ-kokumi肽的生产应用等方面进行了综述,以期为γ-kokumi肽相关增味产品的开发以及GGT酶法合成提供一定的参考。

关键词: γ-kokumi肽, 结构特征, γ-谷氨酰转肽酶, 酶法合成

Abstract: Kokumi is one of the sixth basic taste candidates, whose addition to food can enhance the thickness, mouthfulness, continuity and harmony of taste. As a result, kokumi-related flavor products have a great potential to be developed and marketed. γ-Glutamyl peptides with kokumi flavor are an important class of kokumi substances, also called γ-kokumi peptides, composed of γ-glutamyl and amino acid/polypeptide, which could be synthesized by γ-glutamyl transpeptidase (GGT). In this paper, the structural characteristics and food sources of γ-kokumi peptides and the methods used for their taste evaluation and preparation, as well as the structural characteristics, catalytic mechanism and application for the synthesis of γ-kokumi peptides of GGT are reviewed, which will provide a basis for the development of γ-kokumi peptide-related flavor products and their enzymatic synthesis with GGT.

Key words: γ-kokumi peptides, structural characteristics, γ-glutamyl transpeptidase, enzymatic synthesis

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