食品科学 ›› 2020, Vol. 41 ›› Issue (6): 43-50.doi: 10.7506/spkx1002-6630-20190121-241

• 食品化学 • 上一篇    下一篇

酸解法与酶解法调控右旋糖酐分子质量的比较

黄瑞杰,李媚,廖安平,蓝平,钟磊,覃琴,蓝丽红,王雪娇,韦丽明,甘兰芳   

  1. (广西民族大学化学化工学院,广西多糖材料及改性重点实验室,广西 南宁 530006)
  • 出版日期:2020-03-25 发布日期:2020-03-23
  • 基金资助:
    广西生物多糖分离纯化及改性研究平台建设项目(桂科ZY18076005)

Comparison of Acid Hydrolysis and Enzymatic Hydrolysis of Dextran

HUANG Ruijie, LI Mei, LIAO Anping, LAN Ping, ZHONG Lei, QIN Qin, LAN Lihong, WANG Xuejiao, WEI Liming, GAN Lanfang   

  1. (Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China)
  • Online:2020-03-25 Published:2020-03-23

摘要: 为得到特定分子质量的右旋糖酐,采用右旋糖酐酶对右旋糖酐的分子质量和分子质量分布进行调控,并与传统的酸解法进行对比。结果表明:酶解法和酸解法均可调控右旋糖酐的分子质量,产物分布系数都在合理的范围,产物均一性较好;酶解法反应温度为50 ℃左右,反应条件温和,反应速率快,无氯离子残留;酸解法反应温度在90 ℃左右,反应速率快,但需耗费酸碱,能耗高,污染大,氯离子残留达到5.89%。因此右旋糖酐酶法与酸法相比能耗低,污染小,无氯离子残留,这为酶法代替酸法生产特定分子质量的右旋糖酐提供了有力的依据。

关键词: 右旋糖酐, 右旋糖酐酶, 酸解法, 酶解法, 重均分子质量

Abstract: In order to obtain dextran with specific molecular mass, dextranase was used to hydrolyze dextran and compared with the traditional acid hydrolysis method. The experimental results showed that both enzymatic hydrolysis and acid hydrolysis could control the molecular mass of dextran, the distribution coefficients of the products were within a reasonable range, with good homogeneity. The enzymatic hydrolysis occurred fast under mild reaction conditions at about 50 ℃, and the products had no chloride ion residue. At about 90 ℃, the acid hydrolysis was carried out at a high reaction rate, but it needed a large amount of acid and alkali, with high energy consumption and serious pollution, and the level of chloride ion residue in the products was 5.89%. Therefore, compared with the acid hydrolysis, the enzymatic hydrolysis had the advantages of lower energy consumption, less pollution and no chloride ion residue, which provides a strong basis for the potential of the enzymatic method as an alternative to the acid method to produce dextran with specific molecular mass.

Key words: dextran, dextranase, acid hydrolysis, enzymatic hydrolysis, weight average molecular mass

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