食品科学 ›› 2020, Vol. 41 ›› Issue (6): 139-145.doi: 10.7506/spkx1002-6630-20190130-386

• 生物工程 • 上一篇    下一篇

复合膜固定化酶提高米糠稳定性

于殿宇,陈书曼,王彤,李丹,张雪,吴楠,唐洪琳,秦兰霞,姚凯   

  1. (1.东北农业大学食品学院,黑龙江 哈尔滨 150030;2.湖北天星粮油股份有限公司,湖北 随州 441300)
  • 出版日期:2020-03-25 发布日期:2020-03-23
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0401101); 黑龙江省教育厅课题(TSTAU-C2018011;TSTAU-R2018010)

Improved Stability of Rice Bran with Composite Membrane Immobilized Enzyme

YU Dianyu, CHEN Shuman, WANG Tong, LI Dan, ZHANG Xue, WU Nan, TANG Honglin, QIN Lanxia, YAO Kai   

  1. (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Hubei Tianxing Grain and Oil Co. Ltd., Suizhou 441300, China)
  • Online:2020-03-25 Published:2020-03-23

摘要: 利用醋酸纤维素修饰聚四氟乙烯微孔膜,再将木瓜蛋白酶固定在醋酸纤维素-聚四氟乙烯复合膜上,以新鲜米糠为原料,对其进行酶解钝化。以米糠相对脂肪酶活力为指标,在单因素试验的基础上,采用响应面法优化固定化酶膜钝化米糠脂肪酶条件。结果表明,固定化酶膜钝化米糠脂肪酶的最佳条件为环境空气相对湿度72%、钝化温度71 ℃、钝化时间113 min,在此条件下处理后的米糠相对脂肪酶活力下降至35.2%,有效钝化了米糠中的脂肪酶。米糠贮存2 个月后,其相对脂肪酶活力仍保持在36.0%以下,且固定化酶膜重复使用6 次后,其相对酶活力仍在73.0%。因此,本研究认为将固定化酶膜应用于稳定米糠,有效钝化米糠脂肪酶,并且实现固定化酶膜的可循环利用性。

关键词: 复合膜, 固定, 木瓜蛋白酶, 钝化, 脂肪酶活力, 重复使用

Abstract: Cellulose acetate (CA) was used to modify a microporous polytetrafluoroethylene (PTFE) membrane, and papain was immobilized on the CA-PTFE composite membrane. The final aim of this study was to investigate the deactivation of lipase in fresh rice bran using the immobilized enzyme membrane. The conditions for deactivating lipase in fresh rice bran were optimized using a combination of one-factor-at-a-time method and response surface methodology. The relative lipase activity was taken as the response. The results showed that the optimal conditions for deactivation of rice bran lipase were determined as follows: ambient air relative humidity 72%, deactivation temperature 71 ℃, and time 113 min. Under these conditions, the relative lipase activity of rice bran decreased to 35.2% indicating good deactivation efficiency. After storage for two months, the relative lipase activity of rice bran remained below 36.0%, and the immobilized enzyme membrane still retained 73.0% of its initial activity after sixth repeated use. Thus, this study concluded that the immobilized enzyme membrane could be repeatedly applied to stabilize rice bran by effectively inactivating lipase.

Key words: composite membrane, immobilization, papain, deactivation, lipase activity, repeated use

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