食品科学 ›› 2020, Vol. 41 ›› Issue (6): 179-183.doi: 10.7506/spkx1002-6630-20190221-129

• 生物工程 • 上一篇    下一篇

盐胁迫促进鼠李糖乳杆菌富硒的效果

徐颖,邬淑芳,杨芙莲,余海霞,贺佳璇   

  1. (陕西科技大学食品与生物工程学院,陕西 西安 710021)
  • 出版日期:2020-03-25 发布日期:2020-03-23
  • 基金资助:
    陕西省科技厅农业科技计划项目(2017TSCXL-NY-02-03);陕西省教育厅专项科研计划项目(18JK0094); 陕西科技大学博士科研启动基金项目(2016BJ-21)

Salt Stress Enhanced Selenium Enrichment in Lactobacillus rhamnosus

XU Ying, WU Shufang, YANG Fulian, YU Haixia, HE Jiaxuan   

  1. (School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’an 710021, China)
  • Online:2020-03-25 Published:2020-03-23

摘要: 以鼠李糖乳杆菌(ATCC 53103)为研究对象,在15 mL培养基中添加1 mL 6% NaCl溶液胁迫该菌,亚硒酸钠添加量10 μg/mL,在pH 6.4、温度37 ℃条件下富硒培养,利用电感耦合等离子发射光谱法测定鼠李糖乳杆菌的富硒量和富硒率。结果表明,富硒量最高可达452.43 μg/g,富硒率最高达43.33%,与未胁迫组相比,富硒量和富硒率分别提高了122.72 μg/g和13.17%。用透射电镜观察富硒菌体内外出现黑色微小颗粒,经能谱分析为硒单质,菌体受盐胁迫后富硒量增加,进一步证明了盐胁迫对鼠李糖乳杆菌富硒具有促进作用。

关键词: 鼠李糖乳杆菌, 硒, 生物转化, 盐胁迫

Abstract: Lactobacillus rhamnosus (ATCC 53103) was cultured 37 ℃ in an MRS medium containing 1 mL of 6% NaCl per 15 mL and 10 μg/mL sodium selenite at pH 6.4. The selenium content of the dried biomass was determined to be up to 452.43 μg/g by inductively coupled plasma-atomic emission spectrometry and the selenium enrichment efficiency was calculated as 43.33%. Compared with the unstressed control group, the selenium content and selenium enrichment efficiency were improved by 122.72 μg/g and 13.17%, respectively. Black microparticles were observed both inside and outside the selenium-enriched L. rhamnosus cells by transmission electron microscopy, which was proved to be elemental selenium by energy dispersive X-ray spectroscopy (EDAX). The selenium content increased after salt stress, which further proved that salt stress promoted the selenium enrichment of L. rhamnosus.

Key words: Lactobacillus rhamnosus, selenium, biotransformation, salt stress

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