食品科学 ›› 2020, Vol. 41 ›› Issue (8): 45-51.doi: 10.7506/spkx1002-6630-20190401-014

• 食品化学 • 上一篇    下一篇

辛弗林分子印迹聚合物的制备及其在固相萃取中的应用

李玲,苏学素,张耀海,焦必宁,刘金涛,董家吏   

  1. (1.西南大学化学化工学院,重庆 400715;2.中国农业科学院柑桔研究所(西南大学柑桔研究所),农业农村部柑桔产品质量安全风险评估实验室,重庆 400712)
  • 出版日期:2020-04-25 发布日期:2020-04-20
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFF0201105-2); 国家现代农业(柑桔)产业技术体系建设专项(CARS-26); 国家农产品质量安全风险评估重大专项(GJFP2018004;GJFP2018015)

Preparation and Application of Molecularly Imprinted Polymer in Solid Phase Extraction of Synephrine

LI Ling, SU Xuesu, ZHANG Yaohai, JIAO Bining, LIU Jintao, DONG Jiali   

  1. (1. College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;2. Laboratory of Quality and Safety Risk Assessment for Citrus Products, Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Chinese Academy of Agricultural Sciences (Citrus Research Institute of Southwest University), Chongqing 400712, China)
  • Online:2020-04-25 Published:2020-04-20

摘要: 以辛弗林为模板分子,丙烯酸和丙烯酸羟乙酯为双功能单体,偶氮二异丁腈为引发剂,乙二醇二甲基丙烯酸酯为交联剂,通过沉淀聚合法制备辛弗林分子印迹聚合物。静态吸附法筛选最佳合成条件,测定最佳条件下聚合物的最大吸附量、特异识别性能和吸附机理,通过扫描电子显微镜对聚合物进行形态表征,以合成的分子印迹聚合物作为固相萃取填料,对枳实粗粉中的辛弗林进行提取和纯化。实验结果表明在10 mL乙腈作为致孔剂的条件下,当模板分子与功能单体、引发剂、交联剂物质的量比为1∶4∶2∶20时,分子印迹聚合物形貌良好,对辛弗林具有特异识别性且吸附效果最佳,最大吸附量为228.82 μmol/g。利用分子印迹固相萃取技术对枳实粗粉中的辛弗林进行精制后,辛弗林质量分数由1.93%提高到93.34%,提取率为73.90%。

关键词: 分子印迹, 辛弗林, 固相萃取, 特异识别性

Abstract: In this experiment, molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization using synephrine as a template molecule, acrylic acid and hydroxyethyl acrylate as bifunctional monomers, azobisisobutyronitrile as an initiator, and ethylene glycol dimethacrylate as a crosslinking agent. The synthesis conditions were optimized through static adsorption experiments. The maximum adsorption capacity and specific recognition performance were evaluated under optimized conditions, and the adsorption mechanism was explored. The morphology of the synthesized polymer was characterized by scanning electron microscopy, and its application as a solid phase extraction (SPE) sorbent for separating synephrine extracted from Fructus Aurantii immaturus was investigated. MIPs with good morphology and specific recognition capacity (maximum adsorption capacity of 228.82 μmol/g) for synephrine were obtained using 10 mL of acetonitrile as porogen at a molar ratio of template molecule to functional monomer to initiator to cross-linker of 1:4:2:20. After being purified with the SPE sorbent, the purity of synephrine increased from 1.93% to 93.34% with a recovery of 73.90%.

Key words: molecular imprinting, synephrine, solid phase extraction, specific recognition

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