食品科学 ›› 2020, Vol. 41 ›› Issue (8): 77-82.doi: 10.7506/spkx1002-6630-20181209-106

• 食品化学 • 上一篇    下一篇

铁蛋白-海藻酸钠纳米包埋ACE抑制肽

夏伟荣,李莹,柴智,陈小娥,周剑忠,冯进,方旭波   

  1. (1.浙江海洋大学食品与医药学院,浙江 舟山 316022;2.江苏省农业科学院农产品加工研究所,江苏 南京 210014)
  • 出版日期:2020-04-25 发布日期:2020-04-20
  • 基金资助:
    食品科学与技术国家重点实验室开放课题(SKLF-KF-201712)

Encapsulation of ACE Inhibitory Peptide with Ferritin-Sodium Alginate Nanoparticles

XIA Weirong, LI Ying, CHAI Zhi, CHEN Xiao’e, ZHOU Jianzhong, FENG Jin, FANG Xubo   

  1. (1. School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China;2. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
  • Online:2020-04-25 Published:2020-04-20

摘要: 利用铁蛋白在极酸条件下可逆组装特性和海藻酸钠(sodium alginate,SA)的控释作用,以马脾脱铁铁蛋白(horse spleen apoferritin,HSF)和SA作为纳米载体,包埋血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制肽丙氨酸-组氨酸-亮氨酸-亮氨酸(Ala-His-Leu-Leu,AHLL),以期提高ACE抑制肽AHLL在消化系统中的吸收效果。以包封率为评价指标,优化实验条件。结果显示,纳米粒的最佳制备条件为HSF浓度1~2 μmol/L之间、SA质量浓度10 mg/L、AHLL终质量浓度100~200 μg/mL范围内,此时制备的纳米粒体系包封率较高。运用透射电子显微镜对HSF-AHLL和HSF-SA-AHLL进行结构表征,从表观上呈现出均一的纳米体系。纳米粒的粒径和电位测定结果体现出包埋体系的稳定性。Caco-2细胞单层模型的体外转运实验,证明了纳米体系中的AHLL在消化系统中吸收效果更好。据此铁蛋白和SA可以作为载体被应用在活性肽稳态化保持,也为活性营养成分的吸收提供一条有效途径。

关键词: 马脾脱铁铁蛋白, 血管紧张素转化酶抑制肽, 海藻酸钠, 包埋, 纳米粒, 吸收

Abstract: By taking advantage of the controlled release capacity of sodium alginate (SA) and the reversible assembly characteristics of under extremely acidic conditions, horse spleen apoferritin (HSF) and SA were used as nano-carriers for encapsulation of an angiotensin converting enzyme (ACE) inhibitory peptide, alanine-histidine-leucine-leucine (Ala-His-Leu-Leu, AHLL), aiming to improve the absorption of AHLL in the digestive system. The results showed that the optimal conditions for the preparation of nanoparticles that provided maximum encapsulation efficiency were determined as follows: HSF concentration 1–2 μmol/L, sodium alginate concentration 10 mg/L, and final concentration of AHLL 100–200 μg/mL. The as-prepared nanoparticle system was stable. Preliminary structural representation of HSF-AHLL and HSF-SA-AHLL was performed with transmission electron microscopy, which indicated that a uniform nano-system has been formed. The particle size and potential of the nanoparticles demonstrated the stability of the encapsulation system. Furthermore, AHLL in the nano-system could be well absorbed by the digestive system as revealed by the in vitro transport test uisng Caco-2 cell monolayer model. These results support that HSF-SA could be applied as a nanocarrier for maintaining the homeostasis of bioactive peptides and provide an effective way to improve the absorption of bioactive nutrients.

Key words: horse spleen apoferritin, angiotensin converting enzyme inhibitory peptide, sodium alginate, encapsulation, nanoparticle, absorption

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