食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 生物工程 •    下一篇

食品中肌醇测定用菌株的筛选及检测方法的建立

张宁,范一灵   

  1. 上海市食品药品检验所
  • 收稿日期:2019-03-25 修回日期:2020-03-25 出版日期:2020-05-25 发布日期:2020-05-15
  • 通讯作者: 范一灵 E-mail:tcfyl@163.com
  • 基金资助:
    国家卫生计生委食品安全国家标准修订项目

Screening of strains for determination inositol in food and establishment of measuring method

,yiling fan   

  • Received:2019-03-25 Revised:2020-03-25 Online:2020-05-25 Published:2020-05-15
  • Contact: yiling fan E-mail:tcfyl@163.com

摘要: 摘 要:本文通过肌醇缺陷型酵母菌菌株的生长与培养液中肌醇含量的对应关系,建立了食品中肌醇含量的检测方法。通过比较6株酵母菌对肌醇的生长特异性筛选实验,获得肌醇利用特异性菌株葡萄汁酵母Saccharomyces uvarum CICC 1465。利用该菌进行食品中肌醇的定量检测方法研究,通过对4种食品中肌醇含量的测定,结果显示,本方法获得良好的精密度(相对标准偏差10%以内)、回收率(92.3%~114.3%)及重现性(相对标准偏差10%以内),表明该方法可用于食品中肌醇的定量检测。

关键词: 肌醇, 酵母菌, 检测

Abstract: Abstract: The detection method of inositol in food was established in this paper by the corresponding relationship between the growth of the inositol deficient yeast strain and the content of inositol in medium. The inositol-deficient yeast strain Saccharomyces uvarum CICC 1465 was seleceted from six yeast strains using the growth specific screening method. Then, a quantitative detection method was established by S. uvarum CICC 1465 and showed good precision (RSD less than 10%), recovery (92.3%—114.3%) and reproducibility (RSD less than 10%) in inositol measuring using four kinds of food, which indicated the method can be used for the quantitative determination of inositol in food.

Key words: Inositol, Saccharomyces, Detection

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