食品科学 ›› 2020, Vol. 41 ›› Issue (10): 38-46.doi: 10.7506/spkx1002-6630-20190524-295

• 食品化学 • 上一篇    下一篇

近江牡蛎多糖的结构鉴定及免疫调节能力分析

杨大俏,王锦旭,李来好,杨贤庆,马海霞,岑剑伟,王悦齐   

  1. (1.中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,广东 广州 510300;2.上海海洋大学食品学院,上海 201306;3.韩山师范学院食品工程与生物科技学院,广东 潮州 521041)
  • 出版日期:2020-05-25 发布日期:2020-05-15
  • 基金资助:
    中国水产科学研究院基本科研业务费资助专项(2019ZD1001);广东省公益研究与能力建设专项(2017B020204001); 广东省自然科学基金项目(2016A030313144); 广东省促进经济高质量发展专项资金海洋经济发展项目(GDOE[2019]A25)

Structural Analysis and Immunoregulatory Activity of Polysaccharides from Crassostrea rivularis

YANG Daqiao, WANG Jinxu, LI Laihao, YANG Xianqing, MA Haixia, CEN Jianwei, WANG Yueqi   

  1. (1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;3. School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou 521041, China)
  • Online:2020-05-25 Published:2020-05-15

摘要: 采用分级膜分离、DEAE-52阴离子交换层析法从近江牡蛎(Crassostrea rivularis)中分离纯化得到多糖CRP-1、CRP-2、CRP-3。采用高效凝胶渗透色谱法结合葡聚糖凝胶Sephadex G-100分离多糖,测定其分子质量及纯度;利用糖基基团测定、傅里叶变换红外光谱、高效液相色谱等方法对其结构进行解析。结果显示:CRP-1的总糖质量分数为(32.40±0.89)%,分子质量为124.5 kDa,是组分均一的多糖,由甘露糖、核糖、氨基葡萄糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、氨基半乳糖、半乳糖、阿拉伯糖组成,9 种单糖的物质的量比为5.55∶0.32∶9.08∶0.47∶0.66∶48.13∶3.57∶8.53∶0.30;CRP-2的总糖质量分数为(78.50±1.90)%,分子质量为67.0 kDa,是由甘露糖、核糖、氨基葡萄糖、葡萄糖、氨基半乳糖、半乳糖组成,6 种单糖的物质的量比为7.79∶0.41∶12.69∶32.08∶4.98∶15.92。CRP-3的总糖质量分数为(73.46±2.19)%,分子质量为8.3 kDa,是由氨基葡萄糖、半乳糖醛酸、葡萄糖组成,3 种单糖的物质的量比为8.91∶5.71∶10.15。傅里叶变换红外光谱分析结果显示:CRP-1为多醛基吡喃环糖苷的多糖化合物;CRP-2为多酮基不饱和多糖化合物;CRP-3为多酮基含羧基多不饱和多糖化合物。以小鼠巨噬细胞系RAW264.7为模型,证明近江牡蛎多糖CRP-1、CRP-2、CRP-3具有免疫调节活性。

关键词: 近江牡蛎, 多糖, 结构, 免疫调节

Abstract: By fractional ultrafiltration followed by DEAE-52 anion-exchange column chromatography, an enzymatic hydrolysate of Crassostrea rivularis was fractionated into three polysaccharides named CRP-1, CRP-2 and CRP-3. The molecular masses and purity of the polysaccharides were determined by high performance gel permeation chromatography combined with Sephadex G-100 column chromatography, and their structures were elucidated by determining glycosyl groups as well as using high performance liquid chromatography and Fourier transform infrared (FTIR) spectroscopy. The results showed that the total sugar content of CRP-1 was (32.40 ± 0.89)% with a molecular mass of 124.5 kDa. It was composed of mannose, ribose, glucosamine, glucuronic acid, galacturonic acid, glucose, galactosamine, galactose, arabinose, with a molar ratio of 5.55:0.32:9.08:0.47:0.66:48.13:3.57:8.53:0.30. The total sugar content of CRP-2 was (78.50 ± 1.90)% with a molecular mass of 67.0 kDa. CRP-2 was composed of mannose, ribose, glucosamine, glucose, galactose and galactose with a molar ratio of 7.79:0.41:12.69:32.08:4.98:15.92. The total sugar content of CRP-3 was (73.46 ± 2.19)%, with a molecular mass of 8.3 kDa. It was composed of glucosamine, galactose acid and glucose with a molar ratio 8.91:5.71:10.15. FTIR analysis showed that CRP-1 was a polyaldehyde pyranoside. CRP-2 was an unsaturated polyhydroxyketone while CRP-3 was a polyunsaturated polyhydroxyketone containing carboxyl groups. All the polysaccharides displayed immunomodulatory activity in mouse macrophage line RAW264.7.

Key words: Crassostrea rivularis, polysaccharides, structure, immunoregulatory activity

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