食品科学 ›› 2020, Vol. 41 ›› Issue (10): 88-93.doi: 10.7506/spkx1002-6630-20190324-305

• 生物工程 • 上一篇    下一篇

食品中肌醇测定用菌株的筛选及检测方法建立和应用

张宁,范迪,杨燕,王赢,徐琼,崔学文,房玉国,闻宏亮,秦峰,刘浩,范一灵   

  1. (1.国家药品监督管理局药品微生物检测技术重点实验室,上海市食品药品检验所,上海 201203;2.上海质量监督检验技术研究院,上海 200233;3.上海海关,上海 200135;4.四川省食品药品检验检测院,四川 成都 611731;5.国家乳制品质量监督检验中心,黑龙江 哈尔滨 150086)
  • 出版日期:2020-05-25 发布日期:2020-05-15
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFC1603900); 国家卫生计生委食品安全国家标准修订项目(spaq-2017-066)

Screening of Strains for Determination of Inositol in Food and Establishment and Application of an Analytical Method

ZHANG Ning, FAN Di, YANG Yan, WANG Ying, XU Qiong, CUI Xuewen, FANG Yuguo, WEN Hongliang, QIN Feng, LIU Hao, FAN Yiling   

  1. (1. Key Laboratory for Testing Technology of Pharmaceutical Microbiology, National Medical Products Administration, Shanghai Institute for Food and Drug Control, Shanghai 201203, China; 2. Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China; 3. Shanghai Customs, Shanghai 200135, China; 4. Sichuan Institute for Food and Drug Control, Chengdu 611731, China; 5. National Dairy Engineering and Technical Research Center, Harbin 150086, China)
  • Online:2020-05-25 Published:2020-05-15

摘要: 基于肌醇缺陷型酵母菌菌株的生长与培养液中肌醇含量的对应关系,建立食品中肌醇含量的检测方法。通过比较6 株酵母菌对肌醇的生长特异性筛选实验,获得肌醇利用特异性菌株葡萄汁酵母Saccharomyces uvarum CICC 1465。利用该菌进行食品中肌醇的定量检测方法研究,通过对4 种食品中肌醇含量的测定,结果显示,本方法获得良好的精密度(相对标准偏差10%以内)、回收率(92.3%~114.3%)及重现性(相对标准偏差10%以内),表明该方法可用于食品中肌醇的定量检测。

关键词: 肌醇, 酵母菌, 检测

Abstract: In this study, a method for detecting inositol in food samples was established based on the relationship between the growth of an inositol-deficient yeast strain and the content of inositol in its culture broth. The inositol-deficient yeast Saccharomyces uvarum CICC 1465 was selected from six yeast strains based on its growth specificity for inositol. When it was employed to determine inositol contents in four kinds of foods, the method showed good precision with relative standard deviation (RSD) less than 10%, recoveries (92.3%-114.3%) and reproducibility (RSD less than 10%), indicating that the developed method can be used for the quantitative determination of inositol in food samples.

Key words: inositol, Saccharomyces, detection

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