食品科学 ›› 2020, Vol. 41 ›› Issue (12): 22-27.doi: 10.7506/spkx1002-6630-20190611-115

• 食品化学 • 上一篇    下一篇

碱提条件对麸皮阿拉伯木聚糖组成、理化性质、流变学特性的影响

鲁振杰,李娟,陈正行,李诚,王莉,李亚男   

  1. (江南大学食品学院,江南大学粮食发酵工艺与技术国家工程实验室,江苏 无锡 214122)
  • 出版日期:2020-06-25 发布日期:2020-06-22
  • 基金资助:
    国家食品科学与工程一流学科建设项目(JUFSTR20180203)

Effects of Alkali Extraction Conditions on Monosaccharide Composition, Physicochemical Properties and Rheological Properties of Arabinoxylan from Wheat Bran

LU Zhenjie, LI Juan, CHEN Zhengxing, LI Cheng, WANG Li, LI Yanan   

  1. (National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
  • Online:2020-06-25 Published:2020-06-22

摘要: 以碱提取法制备小麦麸皮阿拉伯木聚糖,采用高效液相色谱法、高效凝胶过滤色谱法和流变分析方法考察不同NaOH浓度、提取温度和提取时间对阿拉伯木聚糖的单糖组成、分子质量分布和黏度的影响。结果表明,随NaOH浓度的增加,阿拉伯木聚糖的纯度呈现先下降后上升的趋势,而得率则先上升后下降;随提取温度和时间的增加,阿拉伯木聚糖的纯度和得率均先增加后趋于平衡。在NaOH浓度、提取温度和提取时间分别为0.5 mol/L、85 ℃和120 min条件下所得阿拉伯木聚糖的纯度和提取得率分别为88.72%和32.8%。阿拉伯木聚糖的分子质量随提取温度的升高而明显减小;溶液的黏度随NaOH浓度的增加明显增大,随提取温度和时间的增加而显著减小。结果表明,不同碱提取条件所得阿拉伯木聚糖的性质差异较大,本实验可为麦麸阿拉伯木聚糖的碱提取和应用研究提供一定理论支持。

关键词: 小麦麸皮, 碱提阿拉伯木聚糖, 单糖组成, 分子质量, 黏度

Abstract: In this paper, arabinoxylan was prepared from wheat bran by an alkali extraction method. The influences of NaOH concentration, extraction temperature and time on the monosaccharide composition, molecular mas distribution and viscosity of arabinoxylan were investigated by high-performance liquid chromatography, high performance gel filtration chromatography and rheological analysis. The results showed that with increasing alkali concentration, the purity of arabinoxylan decreased first and then increased, while the yield showed the opposite trend. As the extraction temperature and time increased, the purity and yield of arabinoxylan increased until reaching a plateau. Their values were respectively 88.72% and 32.8% under the optimized conditions: NaOH concentration 0.5 mol/L, extraction temperature 85 ℃ and extraction time 120 min. The molecular mass of arabinoxylan decreased significantly with increasing extraction temperature. The viscosity of arabinoxylan solution was positively correlated with NaOH concentration but negatively correlated with extraction temperature and time. The results of this study indicated that arabinoxylans obtained under different alkali extraction conditions were distinct in their properties. Our results in this paper can provide a theoretical basis for the alkali extraction and application of arabinoxylan from wheat bran.

Key words: wheat bran, alkali extraction of arabinoxylan, monosaccharide composition, molecular mass, viscosity

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