食品科学 ›› 2020, Vol. 41 ›› Issue (12): 326-331.doi: 10.7506/spkx1002-6630-20190225-180

• 安全检测 • 上一篇    

食用油中黄曲霉毒素B1快速检测试纸的评价

张威,张文中,郭平,胡重怡,吕小丽   

  1. (江西省食品检验检测研究院,江西 南昌 330001)
  • 收稿日期:2020-06-24 修回日期:2020-06-24 出版日期:2020-06-25 发布日期:2020-06-22

Evaluation of Commercial Rapid Test Strips for Detection of Aflatoxin B1 in Edible Oil

ZHANG Wei, ZHANG Wenzhong, GUO Ping, HU Zhongyi, Lü Xiaoli   

  1. (Jiangxi Institute for Food Control, Nanchang 330001, China)
  • Received:2020-06-24 Revised:2020-06-24 Online:2020-06-25 Published:2020-06-22

摘要: 采用空白基质加标制备盲样,并验证盲样的均匀性和稳定性,对3 个厂家的黄曲霉毒素B1快速检测产品进行评价测试,系统性研究产品评价过程中黄曲霉毒素B1添加量、样品数量等条件对性能指标的影响。结果表明,3 个厂家快速检测产品的灵敏度分别为83%、100%、100%,特异性分别为76.2%、79.3%、67.3%,假阴性率分别为17%、0%、0%,假阳性率分别为23.8%、20.7%、32.7%,相对准确度分别为78.9%、87.6%、80.6%。本研究对添加量设置、结果判定等方面进行了深入讨论,阐明了多种因素对快速检测产品评价的影响,有望推进快速检测评价工作的发展。

关键词: 黄曲霉毒素B1, 食品安全快速检测, 胶体金免疫层析试纸条, 评价

Abstract: To evaluate and test three rapid aflatoxin B1 (AFB1) test strips from different manufacturers, blind samples were prepared by blank matrix addition, and their uniformity and stability were verified in this study. We investigated the effects of AFB1 concentration and the number of samples on performance indicators of the test strips. The results showed that the sensitivities of the three products were 83%, 100% and 100%, respectively, the specificities were 76.2%, 79.3% and 67.3%, respectively, the false negative rates were 17%, 0% and 0%, respectively, the false positive rates were 23.8%, 20.7% and 32.7%, respectively, and the relative accuracies were 78.9%, 87.6% and 80.6%, respectively. We conducted in-depth discussions on AFB1 concentration setting, stability evaluation and result judgment, and clarified that there were many factors affecting the evaluation of these products. This study is expected to be helpful for the evaluation of rapid test products.

Key words: aflatoxin B1, food safety rapid test, colloidal gold immunochromatographic test strip, evaluation

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