食品科学 ›› 2020, Vol. 41 ›› Issue (13): 120-126.doi: 10.7506/spkx1002-6630-20190703-053

• 营养卫生 • 上一篇    下一篇

原花青素对小鼠血脂代谢紊乱与肠道菌群干预的影响

杨昌铭,肖瀛,吴其国,尹志婷,周一鸣,周小理   

  1. (上海应用技术大学香料香精技术与工程学院,上海 201418)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401504)

Preventive Effect of Proanthocyanidin on Gut Microbiome in Dyslipidemic Mice

YANG Changming, XIAO Ying, WU Qiguo, YIN Zhiting, ZHOU Yiming, ZHOU Xiaoli   

  1. (School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 目的:通过建立高脂膳食诱导小鼠血脂代谢紊乱模型,随后对小鼠进行原花青素B2干预,评价原花青素B2对高脂模型小鼠血脂代谢异常的干预作用,并从肠道菌群方面来探究原花青素B2调节血脂代谢紊乱的机理。方法:将6 周龄C57BL/6小鼠随机分为3 组:对照组、高脂组、原花青素B2干预组,饲养12 周后测定脏器指数、血脂指标、肝组织酶活力以及肝和结肠组织抗氧化活性能力,运用Illumina高通量测序技术,探究原花青素B2与高脂膳食小鼠肠道菌群之间的关系,利用气相色谱-质谱联用仪检测高脂膳食小鼠粪便中短链脂肪酸含量变化情况。结果:原花青素B2可以显著降低血中总胆固醇含量、甘油三酯含量和动脉粥样硬化指数(P<0.05),显著提高肝组织脂蛋白酶活力、肝脂酶活力和总脂酶活力,并显著增强抗氧化活性,显著抑制氧化损伤产物丙二醛含量(P<0.05),运用高通量测序技术从门与属分类水平分析差异菌群发现,原花青素B2可以使高脂模型小鼠肠道菌群拟杆菌门/厚壁菌门比值上升,埃希氏菌-志贺氏杆菌(Escherichia-Shigella)、未分类拟杆菌(Bacteroidales_S24-7_group)、瘤胃球菌(Ruminococcaceae)、双歧杆菌(Bifidobacterium)、拟杆菌(Parabacteroides)等13 种菌属丰度有显著性差异,与高脂组相比较,原花青素B2组占优势的菌属有Romboutsia、毛螺旋菌属(Lachnospiraceae)和拟杆菌(Parabacteroides)等,原花青素B2可以显著增加高脂膳食小鼠粪便中丁酸的含量(P<0.05)。结论:原花青素B2能够改善肠道环境,调节肠道菌群结构从而调节高脂膳食诱导的血脂代谢紊乱。

关键词: 原花青素B2, 血脂代谢, 抗氧化, 肠道菌群

Abstract: The intervening effect of dietary supplementation of proanthocyanidin B2 on dysfunctional blood lipid metabolism was evaluated in a mouse model of hyperlipidemia induced by a high-fat diet, and the underlying mechanism was explored from the perspective of intestinal flora. C57BL/6 mice (5 weeks old) were randomly divided into three groups: control group (A), high-fat diet (HFD) group (B), and proanthocyanidin B2 treatment group (C). After feeding for 12 weeks, visceral organ indices, blood lipids, hepatic enzyme activities and antioxidant capacity in liver and colon tissues were determined, the intestinal flora was analyzed using Illumina high-throughput sequencing technology, and changes in short-chain fatty acid contents in the feces of the mice were detected by gas chromatography-mass spectrometry. The results showed that proanthocyanidin B2 significantly reduced the levels of serum total cholesterol, triglyceride and atherosclerosis index (P < 0.05), significantly increased lipoprotein lipase activity, hepatic lipase activity, total lipase activity, and antioxidant activity, and inhibited malondialdehyde accumulation as an indicator of oxidative damage (P < 0.05). Taxonomic classification of the mouse intestinal microbiome at the phylum and genus levels demonstrated that proanthocyanidin B2 could increase Bacteroidetes/Firmicutes ratio in the intestinal flora of the HFD-fed mice and result in significant differences in the abundance of 13 species of bacteria, including Escherichia-shigella, Bacteroidales_S24-7_group, Ruminococcaceae, Bifidobacterium and Parabacteroides. The dominant bacterial genera in the proanthocyanidin B2 group were Romboutsia, Lachnospiraceae and Parabacteroides. Proanthocyanidin B2 significantly increased the contents of butyric acid in the feces of the HFD-fed mice (P < 0.05). Therefore, proanthocyanidin B2 can regulate the intestinal flora structure, thus preventing high-fat?diet-induced?dyslipidemia.

Key words: proanthocyanidin B2, blood lipid metabolism, antioxidant, intestinal flora

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