仙草多糖对细胞氧化损伤的保护作用

doi:10.7506/spkx1002-6630-20190714-183

食品科学 ›› 2020, Vol. 41 ›› Issue (13): 160-168.doi: 10.7506/spkx1002-6630-20190714-183

仙草多糖对细胞氧化损伤的保护作用

何传波，邓婷，魏好程，吴国宏，上官宇晨，熊何健

（集美大学食品与生物工程学院，福建厦门 361021）

出版日期:2020-07-15 发布日期:2020-07-29
基金资助:
福建省自然科学基金项目（2017J01634）；福建省区域发展项目（2019N3012）

Protective Effect of Polysaccharide from Mesona blumes on Oxidative Damage of Cells

HE Chuanbo, DENG Ting, WEI Haocheng, WU Guohong, SHANGGUAN Yuchen, XIONG Hejian

(College of Food and Biological Engineering, Jimei University, Xiamen 361021, China)

Online:2020-07-15 Published:2020-07-29

摘要/Abstract

摘要： 采用碱液浸提法从仙草中提取粗多糖JMBP-C，通过超滤、离子交换和凝胶柱层析纯化获得一种中性多糖JMBP-N和两种酸性多糖JMBP-A1和JMBP-A2。采用H2O2对人体肝细胞LO2进行氧化损伤，构建LO2细胞的氧化损伤模型。通过测定细胞存活率、乳酸脱氢酶（lactate dehydrogenase，LDH）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）活力和丙二醛（malondialdehyde，MDA）含量探讨JMBP-C、JMBP-A1和JMBP-A2对氧化损伤细胞的保护作用。结果表明，3 种多糖能显著增加氧化损伤细胞的存活率，有效降低LDH的释放量，2 mg/mL时，仙草多糖均能极显著降低损伤细胞LDH活力（P＜0.01），JMBP-A1质量浓度为0.5 mg/mL时，细胞存活率比模型组增加49.47%。仙草多糖能提高GSH-Px、SOD和CAT活力，且呈现一定的量效关系。JMBP-A2质量浓度为0.125 mg/mL时能将GSH-Px活力提高到正常细胞的69.20%，高于阳性对照，2 mg/mL的JMBP-A1将SOD和CAT活力分别恢复到正常对照组的80.32%和88.14%，极显著高于模型组（P＜0.01），效果接近阳性对照。同时，3 种多糖样品均能有效减少MDA生成，0.5 mg/mL的JMBP-A1使受损细胞的MDA含量降低了31.50%，与阳性对照效果相当。综上，仙草多糖可增加细胞内抗氧化酶活性，降低脂质过氧化物生成，有明显的氧化损伤保护作用。

关键词: 仙草多糖, 细胞, 氧化损伤, 抗氧化酶

Abstract: The crude polysaccharide JMBP-C was extracted from Mesona blumes with an alkaline solution. The neutral polysaccharide JMBP-N and the two acidic polysaccharides JMBP-A1 and JMBP-A2 were obtained by fractionating JMBP-C through ultrafiltration, ion exchange and gel column chromatography. Hydrogen peroxide was used to induce oxidative damage to human liver LO2 cells. The protective effects of JMBP-C, JMBP-A1 and JMBP-A2 on oxidative damage in cells were investigated by cell viability, the activities of lactate dehydrogenase (LDH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and the content of malondialdehyde (MDA). The results showed that all three polysaccharides could significantly increase the survival rate of oxidatively damaged cells and effectively reduce the release of LDH. The LDH activity of damaged cells was significantly decreased by each of the polysaccharides at 2 mg/mL (P < 0.01). JMBP-A1 at 500 μg/mL increased the cell survival rate 49.47% compared with the model group. The three polysaccharides could improve the activities of GSH-Px, SOD and CAT in a dose-dependent manner. JMBP-A2 at 0.125 mg/mL increased GSH-Px activity to 69.20% of the normal level, which was higher than that of the positive control. JMBP-A1 at 2 mg/mL could restore the activity of SOD and CAT to 80.32% and 88.14% of the normal group respectively, which was significantly higher than those of the model group (P < 0.01), and the effect was close to that of the positive control group. Moreover, all three polysaccharides could effectively reduce MDA production. JMBP-A1 at 0.5 mg/mL reduced MDA content in damaged cells by 31.50%, and this effect was comparable to that of the positive control. In summary, the polysaccharides from Mesona blumes can increase the activity of antioxidant enzymes and decrease the production of lipid peroxides, suggesting a significant protective effect against oxidative stress-induced cellular damage.

Key words: polysaccharides from Mesona blumes, cell, oxidative damage, antioxidant enzyme

中图分类号:

TS255.2

何传波, 邓婷, 魏好程, 吴国宏, 上官宇晨, 熊何健. 仙草多糖对细胞氧化损伤的保护作用[J]. 食品科学, 2020, 41(13): 160-168.

HE Chuanbo, DENG Ting, WEI Haocheng, WU Guohong, SHANGGUAN Yuchen, XIONG Hejian. Protective Effect of Polysaccharide from Mesona blumes on Oxidative Damage of Cells[J]. FOOD SCIENCE, 2020, 41(13): 160-168.

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仙草多糖对细胞氧化损伤的保护作用

Protective Effect of Polysaccharide from Mesona blumes on Oxidative Damage of Cells

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