食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 专题论述 •    下一篇

抗冻蛋白与冰水界面层相互作用的理论机制研究进展

吴金鸿1,杨丹璐1,周密1,陈旭2,3,蔡茜茜2,施依1,杨傅佳2,汪少芸2,许璟珅1,张恒4   

  1. 1. 上海交通大学农业与生物学院
    2. 福州大学生物科学与工程学院
    3. 福州大学石油化工学院
    4. 安徽国肽生物科技有限公司
  • 收稿日期:2020-04-20 修回日期:2021-02-23 出版日期:2021-06-15 发布日期:2021-06-28
  • 通讯作者: 汪少芸 E-mail:shywang@fzu.edu.cn
  • 基金资助:
    国家自然科学基金海峡重点项目;科技部国家重点研发计划专项;国家自然科学基金面上项目;宁波市公益类科技计划项目

Research Advances of the Theoretical Mechanism about the Interaction between Antifreeze Proteins and Ice-Water Interface Layer

1, 1, 1,Xu Chen 1, 1,Fujia Yang1, 1, 1   

  • Received:2020-04-20 Revised:2021-02-23 Online:2021-06-15 Published:2021-06-28

摘要: 抗冻蛋白(AFPs)具有热滞活性和重结晶抑制活性,并在冷冻食品品质调控、细菌低温保护、植物霜冻保护等领域具有广泛的应用价值。本文围绕种类和结构复杂的AFPs 在低温冷冻环境下如何通过调控冰水界面层结构,与冰晶结合或相互作用从而实现其抑制冰晶生长,产生热滞活性和重结晶抑制活性的理论问题,系统综述了吸附抑制模型、偶极子-偶极子模型、晶格匹配与占有模型、刚体能量模型、包合物锚定模型、亲和相互作用偶联团聚模型等理论模型原理和特点,揭示了AFPs通过调控冰水界面层结构产生结合或相互作用原理及其驱动力作用特点,为解析AFPs抗冻活性机理及其筛选、食品工业应用提供理论参考。

关键词: 抗冻蛋白, 抗冻机制, 冰水界面层, 理论模型

Abstract: Antifreeze proteins (AFPs) have thermal hysteresis activity and recrystallization inhibition activity, and have wide and valuable applications in the quality control of frozen food, low temperature protection of bacteria, plant frost protection, and so on. This paper focuses on the theoretical problem of how AFPs with various species and structure can inhibit the growth of ice crystals, generate thermal hysteresis and recrystallization inhibitory activities by regulating the structure of ice water interfacial layer and interacting with ice crystals in the cryogenic freezing environment. This review systematically describes the principles and characteristics of some theoretical models, including adsorption-inhibition model, dipole-dipole model, lattice matching and possession model, rigid body energy model, anchored clathrate model, affinity interaction coupling and aggregation mode. This review reveals the principle of binding or interaction of AFPs with ice-water system by regulating the structure of the ice-water interface layer, and its driving force characteristics, which provides theoretical guides for the elaboration of antifreeze activity mechanism, screening and food industrial application of AFPs.

Key words: antifreeze protein, antifreeze activity mechanism, ice-water interface layer, theoretical model

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