生物合成天然2-苯乙醇细菌的筛选及合成途径分析

摘要/Abstract

摘要： 天然2-苯乙醇是一种具有玫瑰风味的芳香醇，广泛应用于食品、药品和化妆品等领域。目前微生物合成2-苯乙醇主要利用酵母以L-苯丙氨酸为底物通过艾氏途径获得。本研究从上海国家森林公园土壤样本中筛选到一株菌株，经过生理生化鉴定和16S rDNA序列分析，该菌属肠杆菌属并命名为Enterobacter sp. strain MF024。Enterobacter sp. strain MF024全基因组测序结果表明该菌株包含从头合成途径和艾氏途径合成2-苯乙醇所有关键酶的编码基因。分别以葡萄糖、L-苯丙氨酸为底物进行生物转化实验，并以苯乙醛、苯丙酮酸等合成途径中间产物为底物进行验证，气相色谱-质谱法、红外光谱分析结果进一步说明Enterobacter sp. strain MF024具备两种2-苯乙醇合成途径且该菌利用从头合成途径和艾氏途径产2-苯乙醇产量分别达到0.56 g/L，1.15 g/L。该菌株以葡萄糖为碳源生物合成2-苯乙醇极具应用前景。

关键词: 肠杆菌, 2-苯乙醇, 艾氏途径, 从头合成途径

Abstract: Natural 2-phenylethanol is an aromatic alcohol with rose flavor. It is widely used in food, medicine and cosmetics. Currently, the 2-phenylethanol from microorganisms is mainly obtained by yeasts. The 2-phenylethanol is produced by L-phenylalanine as the substrate through the Ehrlich pathway. In this study, a bacterial strain was isolated from the soil samples at the Gulf Forest Park in Shanghai. Biochemical identification and 16S rDNA sequence analysis indicated that the strain was Enterobacter bacteria and designated as Enterobacter sp. Strain MF024. Whole-Genome sequences analysis showed that this strain had all the key enzyme encoding genes in both Ehrlich pathway and the de novo synthesis pathway for the synthesis of 2-phenylethanol. Then, biotransformation experiments were carried out using glucose and L-phenylalanine as the substrates, respectively. The key intermediates in the synthetic pathways such as phenylacetaldehyde and phenylpyruvate were further analyzed. The Enterobacter sp. strain MF024 contained both the 2-phenylethanol synthesis pathways according to the results from gas chromatography-mass spectrometry and infrared spectrometry. The 2-phenylethanol yields reached 0.56 g/L in de novo synthesis pathway and 1.15 g/L in Ehrlich pathway, respectively. This strain has potential application value in the prodution of natural 2-phenylethanol as demonstrated by using glucose as a carbon source.

Key words: Enterobacter, 2-phenylethanol, Ehrlich pathway, de novo synthesis

中图分类号:

Q939.97

荣绍丰伍进管世敏蔡宝国张硕李茜茜. 生物合成天然2-苯乙醇细菌的筛选及合成途径分析[J]. 食品科学, 0, (): 0-0.

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