食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 24-27.doi: 10.7506/spkx1002-6630-201102006

• 工艺技术 • 上一篇    下一篇

超声波提取雪莲薯多糖工艺优化及其对羟自由基的清除

吕明生1,王淑军1,房耀维1,刘姝1,徐兴权2,李秋敏1   

  1. 1. 淮海工学院食品工程学院
    2. 连云港市农业科学院
  • 收稿日期:2010-03-02 修回日期:2010-11-28 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 吕明生 E-mail:mingshenglu@hotmail.com
  • 基金资助:

    江苏省科技厅工业支撑计划项目(BE2009095)

Polysaccharides from Yacon: Optimization of Ultrasound-assisted Extraction and Hydroxyl Free Radical Scavenging Evaluation

LU Ming-sheng1,WANG Shu-jun1,FANG Yao-wei1,LIU Shu1,XU Xing-quan2,LI Qiu-min1   

  1. 1. School of Food Engineering, Huaihai Institute of Technology, Lianyungang 222005, China;
    2. Lianyungang Academy of Agricultural Sciences, Lianyungang 222006, China
  • Received:2010-03-02 Revised:2010-11-28 Online:2011-01-25 Published:2011-01-10
  • Contact: LU Ming-sheng1 E-mail:mingshenglu@hotmail.com

摘要:

以雪莲薯为原料,通过单因素和正交试验研究超声波提取多糖的工艺条件。结果表明,最佳提取工艺条件为提取时间15min、提取温度50℃、超声波功率80W、料液比1:30(g/mL),雪莲薯多糖得率为53.3%。提取效果影响大小的先后顺序为提取时间>提取温度>提取功率>料液比。未脱蛋白多糖对羟自由基有较强的清除作用,IC50 为1.82mg/mL。脱蛋白后多糖对羟自由基清除作用得到显著的提高,IC50 为0.085mg/mL,比未脱蛋白多糖的清除作用提高了20 余倍。

关键词: 雪莲薯, 超声波, 多糖, 提取工艺, 羟自由基

Abstract:

In this study, the process conditions for extracting polysaccharides from yacon fruits with the assistance of ultrasound were optimized by single factor and orthogonal array design methods. The optimal extraction process conditions were determined as follows: ultrasound power, 80 W; extraction temperature, 50 ℃; and material/liquid ratio, 1:30 (g/mL) for an extraction duration of 15 min. Under these conditions, the extraction yield of polysaccharides from yacon fruits was 53.3%. The decreasing order of importance of the four process conditions affecting polysaccharide extraction was extraction duration, extraction temperature, ultrasound power and material/liquid ratio. The crude polysaccharides obtained had strong hydroxyl free radical scavenging effect, with an IC50 of 1.82 mg/mL, which was more than 20-fold increased after protein removal, revealing an IC50 of 0.085 mg/mL.

Key words: yacon, ultrasound, polysaccharide, extraction process, hydroxyl free radicals

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