食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 32-35.doi: 10.7506/spkx1002-6630-201102008

• 工艺技术 • 上一篇    下一篇

杜仲粕中桃叶珊瑚苷的分离和纯化

李辉1,2,文赤夫1,2,李亚男1,彭玉丹1,汪兰1,张永康1,2   

  1. 1. 吉首大学生物资源与环境科学学院 2. 吉首大学 植物资源保护与利用省级重点实验
  • 收稿日期:2010-04-02 修回日期:2010-12-01 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 李辉 E-mail:lihuijsdx@163.com
  • 基金资助:

    湖南省教育厅优秀青年项目(06B076)

Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal

LI Hui1,2,WEN Chi-fu1,2,LI Ya-nan1,PENG Yu-dan1,WANG Lan1,ZHANG Yong-kang1,2   

  1. 1. College of Biology and Environmental Science, Jishou University, Jishou 416000, China;
    2. Hunan Provincial Key Laboratory of Plant Resource Protection and Exploitation, Jishou University, Jishou 416000, China
  • Received:2010-04-02 Revised:2010-12-01 Online:2011-01-25 Published:2011-01-10
  • Contact: LI Hui E-mail:lihuijsdx@163.com

摘要:

为研究大孔树脂对桃叶珊瑚苷的吸附与解吸性能及分离纯化效果,通过静态吸附与解吸实验从12 种大孔树脂中筛选高性能树脂,用树脂进行柱层析分离目标产物并优化分离条件。结果表明:大孔树脂SIPI-7、HZ-820和S-8 对目标分子既有高的吸附率,又能有效脱附;当用HZ-820 型树脂进行柱层析分离纯化桃叶珊瑚苷时,优化分离条件为上样量0.5g/g(以生药质量:树脂质量计)、乙醇- 水- 冰醋酸混合溶剂(50:50:3,V/V)为洗脱剂、流速1.0mL/min。在优化条件下,收集含产物浓度高的流分,经浓缩和重结晶后所获产品中桃叶珊瑚苷纯度高达96.82%,且具有可观的产品得率。

关键词: 桃叶珊瑚苷, 分离纯化, 大孔树脂, 杜仲粕

Abstract:

The objective of this study was to develop a macroporous resin adsorption method for separating and purifying aucubin in Eucommia ulmoides seed meal extracted into 80% aqueous ethanol solution. Twelve kinds of macroporous resins were compared for differences in their adsorption and desorption performances towards aucubin, and the results indicated that macroporous resins SIPI-7, HZ-820 and S-8 had better abilities to adsorb and desorb aucubin, among which, HZ-820 was considered to be the best resin due to the highest desorption rate. The optimal conditions for the adsorption and desorption of HZ-820 were found to be: sample loading, 0.5 g/g (weight ratio of Eucommia ulmoides seed meal extracted to HZ-820 resin); desorpion solvent, 50% aqueous ethanol solution containing 3% acetic acid at a flow rate of 1.0 mL/min. The high aucubin eluate fractions obtained under such conditions were pooled, condensed and recrystallized into a considerable amount of product containing 96.82% of aucubin.

Key words: aucubin, separation and purification, macroporous resin, Eucommia ulmoides seeds

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