食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 39-42.doi: 10.7506/spkx1002-6630-201102010

• 工艺技术 • 上一篇    下一篇

猪胃黏膜中胃膜素提取工艺的优化

董娜,董文宾,田颖   

  1. 陕西科技大学生命科学与工程学院
  • 收稿日期:2010-04-13 修回日期:2010-11-29 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 董娜 E-mail:nadong2010@163.com
  • 基金资助:

    陕西农产品加工技术研究院应用基础研究项目(NCY-080105)

Optimization of the Extraction Process for Gastric Mucin from Porcine Gastric Mucosa

DONG Na,DONG Wen-bin,TIAN Ying   

  1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China
  • Received:2010-04-13 Revised:2010-11-29 Online:2011-01-25 Published:2011-01-10
  • Contact: DONG Na E-mail:nadong2010@163.com

摘要:

以猪胃黏膜为原料,优化胃膜素的提取工艺。以盐酸溶液为提取液,选取液料比、盐酸体积分数、提取温度、提取时间4 个因素,对胃膜素得率进行评价,在单因素试验基础上,采用二次回归正交旋转组合设计优化胃膜素提取的工艺条件。结果表明:最佳提取工艺条件为液料比0.61:1(mL/g)、盐酸体积分数5.4%、提取温度 44.9℃、提取时间2.0h;在此条件下,胃膜素得率为3.74%,所得产品中总氮质量分数为8.7%,还原性物质质量分数为26. 3%,符合《化药部颁标准》规定的质量标准,说明该优化方法可行。

关键词: 胃膜素, 提取, 优化

Abstract:

This paper presents an optimized extraction process for gastric mucin from porcine gastric mucosa with aqueous hydrochloric acid solution. A series of single factor experiments were carried out to evaluate the effects of material/liquid ratio, hydrochloric acid concentration, extraction temperature and length of extraction time on gastric mucin yield, and the four process conditions were optimized by quadratic orthogonal rotary combination design based on this. The optimal process conditions for the extraction of gastric mucin were determined as follows: liquid/material ratio, 0.61:1 (mL/g); hydrochloric acid concentration, 5.4%; extraction temperature, 44.9 ℃; and extraction duration 2.0 h. Under these conditions, the yield of gastric mucin was 3.74%, and the product obtained contained 8.7% of total nitrogen and 26.3% of reducing substances and met the quality requirements stipulated by the Ministerial Standards of Chemical Medicine. Thus, the optimized extraction process is feasible for the preparation of gastric mucin.

Key words: gastric mucin, extraction, optimization

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