食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 53-56.doi: 10.7506/spkx1002-6630-201102013

• 工艺技术 • 上一篇    下一篇

高粱坚轴黑粉菌多糖的提取与纯化

于淼,石太渊,马涛,李莉峰,迟吉捷,王 琛,韩艳秋,高雅   

  1. 辽宁省农业科学院食品与加工研究所
  • 收稿日期:2010-03-17 修回日期:2010-10-30 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 于淼 E-mail:jannytiti@163.com
  • 基金资助:

    沈阳市自然科学基金项目(1071131-3-00)

Extraction and Purification of Polysaccharides from Sphacelotheca sorghi (Link) Clint

YU Miao,SHI Tai-yuan,MA Tao,LI Li-feng,CHI Ji-jie,WANG Chen,HAN Yan-qiu,GAO Ya   

  1. (Food and Processing Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China)
  • Received:2010-03-17 Revised:2010-10-30 Online:2011-01-25 Published:2011-01-10
  • Contact: YU Miao E-mail:jannytiti@163.com

摘要:

对高粱坚轴黑粉菌多糖的提取工艺进行研究,探讨浸提次数、时间、料液比和温度对多糖得率的影响,在单因素试验的基础上,通过正交试验确定最佳提取工艺条件。结果表明:温度对多糖得率的影响最大,其次为料液比、提取次数,提取时间的影响最小。高粱坚轴黑粉菌多糖提取的最优条件为料液比1:20(g/mL)、80℃水浴、浸提3 次、每次浸提2h,其水溶性粗多糖提取率约为7%。并采用鞣酸、活性炭结合等方法纯化粗多糖,得到的多糖纯度为75.63%。

关键词: 高粱坚轴黑粉菌, 多糖, 提取

Abstract:

The process of polysaccharide extraction from Sphacelotheca sorghi (Link) Clint was investigated in this study. The effects of extraction number and temperature, length of extraction time, and material/liquid ratio on polysaccharide yield were explored, and the optimization of the four process conciliations was achieved by orthogonal array design method based on single factor experiments. Extraction temperature was found to have the most important effect on polysaccharide yield, sequentially followed by material/liquid ratio, extraction number and length of extraction time. The optimal extraction process for polysaccharides was Sphacelotheca sorghi (Link) Clint was extracted three times for 2 h each time with a 20-fold volume of water in 80 ℃ water bath, and the resulting yield of crude water-soluble polysaccharides was approximately 7%. The purification of the crude product obtained was performed by using tannic acid and activated carbon together, and as a result, its purity was increased to 75.63%.

Key words: Sphacelotheca sorghi (Link) Clint, polysaccharide, extraction

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