食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 66-70.doi: 10.7506/spkx1002-6630-201102016

• 工艺技术 • 上一篇    下一篇

响应面法优化刺槐花多酚的超声提取工艺

王晓阳,唐琳*,赵垒   

  1. 山东师范大学生命科学学院,山东济南 250014
  • 收稿日期:2010-03-05 修回日期:2010-12-13 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 唐琳 E-mail:tanglin@sdnu.edu.cn

Optimization of Ultrasound-assisted Extraction of Phenolic Compounds from Robinia pseudoacacia L. Flowers by Response Surface Methodology

WANG Xiao-yang,TANG Lin*,ZHAO Lei   

  1. College of Life Science, Shandong Normal University, Jinan 250014, China
  • Received:2010-03-05 Revised:2010-12-13 Online:2011-01-25 Published:2011-01-10
  • Contact: TANG Lin E-mail:tanglin@sdnu.edu.cn

摘要:

目的:利用响应面法对刺槐花多酚的提取工艺进行优化。方法:在单因素试验的基础上,选择温度、超声功率、超声时间、液料比为自变量,多酚提取得率为响应值,利用响应面分析法,研究各自变量交互作用及其对多酚得率的影响,模拟得到二次多项式回归方程的预测模型。结果:刺槐多酚类化合物的提取工艺为温度35℃、超声功率110W、超声时间36min、液料比20:1(mL/g)。结论:在最佳工艺条件下多酚得率为7.891%。

关键词: 刺槐花, 多酚, 响应面法

Abstract:

Objective: The optimization of the extraction process of phenolic compounds from Robinia pseudoacacia L.flowers under ultrasound assistance was achieved by response surface methodology to maximize phenolic extraction yield. Methods: On the basis of single-factor experiments, Box-Behnken central composite design (CCD) involving four variables at three levels was utilized to set up a quadratic polynomial regression equation for phenolic extraction yield as function of extraction temperature, ultrasound power, length of ultrasound treatment time and material/liquid ratio, and the pairwise interactive effects of the above four variables on their function were investigated by response surface analysis. Results: The optimum conditions for extracting phenolic compounds from Robinia pseudoacacia L.flowers were found to be: 36 min ultrasound treatment at 110 W power for extracting the raw material with a 20-fold volume of 60% aqueous ethanol solution at 35 ℃. Under these optimum conditions, the yield of polyphenols was 7.891%.

Key words: Robinia pseudoacacia L. flowers, phenolic compounds, response surface methodology

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