响应曲面法优化超声波提取沙葱籽多糖工艺

doi:10.7506/spkx1002-6630-201102023

食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 98-103.doi: 10.7506/spkx1002-6630-201102023

响应曲面法优化超声波提取沙葱籽多糖工艺

张君萍^1,2，侯喜林^1,2,*，董海艳^1,2，俞阗¹，马志虎^1,2

1.南京农业大学作物遗传与种质创新国家重点实验室 2.农业部南方蔬菜遗传改良重点开放实验室

收稿日期:2010-04-30 出版日期:2011-01-25 发布日期:2011-01-10
通讯作者: 侯喜林 E-mail:hxl@njau.edu.cn
基金资助:
国家公益性行业(农业)科研项目(200903018)

Process Optimization for the Ultrasonic-assisted Extraction of Allium mongolicum Regel Seed Polysaccharides Using Response Surface Methodology

ZHANG Jun-ping1,2，HOU Xi-lin1,2,*，DONG Hai-yan1,2，YU Tian1，MA Zhi-hu1,2

1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China；
2. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, China

Received:2010-04-30 Online:2011-01-25 Published:2011-01-10
Contact: HOU Xi-lin E-mail:hxl@njau.edu.cn

摘要/Abstract

摘要：

目的：以野生沙葱种子经超临界二氧化碳萃取脱脂后的滤渣为材料，利用响应面法优化超声波提取沙葱籽多糖工艺。方法：在单因素试验的基础上，以水料比、提取温度、提取时间为影响因素，应用Box-Behnken中心组合方法进行三因素三水平试验，以多糖得率为响应值，进行响应面分析。结果：沙葱籽多糖提取的最佳工艺条件为水料比48.0:1(mL/g)、提取温度73.1℃、提取时间1.6h、超声波功率300W、提取次数2 次，沙葱籽多糖得率预测值为7.59%，验证值为7.47%，与预测值的相对误差为1.58%。结论：超声波法提取沙葱籽多糖能大大缩短提取时间，且方法简便、准确、高效。

关键词: 响应面法, 沙葱籽, 多糖, 超声波提取

Abstract:

Objective: The ultrasonic-assisted extraction process for polysaccharides from the residue of Allium mongolicum Regel seeds after the oil of extraction with supercritical carbon dioxide was optimized using response surface methodology. Methods: On the basis of single factor experiments, a three-factor, three-level Box-Behnken central composite design (CCD) was utilized for mathematical modeling for polysaccharide yield as a response to extraction temperature and time as well as material/water ratio, followed by response surface analysis of the relationship between the function and the three variables. Results: The optimum conditions for the extraction of Allium mongolicum Regel seed polysaccharides were as follows: water/ material ratio, 48.0:1; ultrasonic power, 300 W; extraction temperature, 73.1 ℃; extraction time, 1.6 h; and extraction number, 2. Under such conditions, the model-predicted and experimental values of polysaccharide yield were 7.59% and 7.47%, respectively, revealing 1.58% relative error between them. Conclusion: Ultrasonic treatment can substantially shorten the time required for polysaccharide extraction, and the developed extraction process is simple, reliable and highly efficient.

Key words: response surface methodology, Allium mongolicum Regel seeds, polysaccharides, ultrasonic-assisted extraction

中图分类号:

S633.9

张君萍，侯喜林，董海艳，俞阗，马志虎. 响应曲面法优化超声波提取沙葱籽多糖工艺[J]. 食品科学, 2011, 32(2 ): 98-103.

ZHANG Jun-ping1,2，HOU Xi-lin1,2,*，DONG Hai-yan1,2，YU Tian1，MA Zhi-hu1,2. Process Optimization for the Ultrasonic-assisted Extraction of Allium mongolicum Regel Seed Polysaccharides Using Response Surface Methodology[J]. FOOD SCIENCE, 2011, 32(2 ): 98-103.

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响应曲面法优化超声波提取沙葱籽多糖工艺

Process Optimization for the Ultrasonic-assisted Extraction of Allium mongolicum Regel Seed Polysaccharides Using Response Surface Methodology

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