食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 111-114.doi: 10.7506/spkx1002-6630-201102026

• 工艺技术 • 上一篇    下一篇

石榴皮总黄酮的提取工艺及抑制亚硝化反应的研究

吴佳,解成喜*   

  1. 新疆大学理化测试中心
  • 收稿日期:2010-03-22 修回日期:2010-12-20 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 解成喜 E-mail:xiecx@xju.edu.cn

Extraction and Nitrosation Inhibition Activity of Total Flavonoids from Punica granatum L. Peel

WU Jia,XIE Cheng-xi*   

  1. Physical and Chemical Analysis Center, Xinjiang University, Urumqi 830046, China
  • Received:2010-03-22 Revised:2010-12-20 Online:2011-01-25 Published:2011-01-10
  • Contact: XIE Cheng-xi* E-mail:xiecx@xju.edu.cn

摘要:

目的:研究石榴皮总黄酮的提取工艺及黄酮类物质对亚硝化反应的抑制能力。方法:通过正交试验,探讨影响总黄酮提取率的因素范围和最佳组合,并采用分光光度法测定总黄酮提取液对亚硝酸盐清除能力和亚硝胺抑制能力。结果:石榴皮总黄酮的最佳提取工艺条件为乙醇体积分数60%、料液比1:60(g/mL)、提取时间40min、超声电流强度150mA。在最佳提取工艺条件下,石榴皮总黄酮的平均提取率为84.8mg/g,石榴皮总黄酮提取液对亚硝酸钠的最大清除率为81.2%,对亚硝胺合成的最大阻断率为67.0%。结论:石榴皮总黄酮提取液对亚硝化反应的抑制能力较强。

关键词: 石榴皮, 总黄酮, 正交试验, 清除, 阻断

Abstract:

The optimal conditions for total flavonoids extraction from Punica granatum L. peel were studied by orthogonal array design and their abilities to scavenge nitrite and inhibit nitrosamines were tested spectrophotometrically. A maximum extraction yield of 84.8 mg flavonoids/g peel was obtained when a 60-fold volume of 60% aqueous ethanol solution was utilized to extract Punica granatum L. peel for 40 min with the assistance of sonication at 150 mA electric current intensity, and the crude extract exhibited a maximum sodium nitrite scavenging rate of 81.2% and a maximum block rate of 67.0% against the synthesis of nitrosamines, suggesting a strong ability to inhibit nitrosation.

Key words: Punica granatum L. peel, total flavonoids, orthogonal array design, scavenging;blocking

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