食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 132-135.doi: 10.7506/spkx1002-6630-201102031

• 工艺技术 • 上一篇    下一篇

新疆产蜂胶总黄酮的提取工艺优化及GC-MS 分析

木努尔丁·托合尼牙孜1,艾尔肯·艾米尔2,依米提·热合曼1 ,*   

  1. 1. 新疆大学生命科学与技术学院
    2. 新疆大学理化测试中心
  • 收稿日期:2009-11-18 修回日期:2010-10-03 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 依米提·热合曼 E-mail:yimitrahman@gmail.com
  • 基金资助:

    新疆大学博士启动基金项目(BS060104);国家自然科学基金项目(30860076)

Optimization of Extraction Process for Total Flavonoids from Xinjiang Propolis and Its Flavonoid Composition as Analyzed by GC-MS

Munuerding TOHENIYAZI1,Aierken AIMIER2,Yimit RAHMAN1,*   

  1. 1. College of Life Science and Technology, Xinjiang University,Urumqi 830046, China;
    2. Physical and Chemical Analysis Center, Xinjiang University,Urumqi 830046, China
  • Received:2009-11-18 Revised:2010-10-03 Online:2011-01-25 Published:2011-01-10
  • Contact: Yimit RAHMAN1,* E-mail:yimitrahman@gmail.com

摘要:

目的:确定新疆产蜂胶中总黄酮的最优化提取工艺,测定新疆产蜂胶中总黄酮的含量,并鉴定总黄酮的组成。方法:采用单因素结合正交试验的方法对新疆蜂胶中总黄酮的提取工艺进行优化,并以芦丁为对照品,用分光光度法在最大吸收波长502nm 处对其含量进行测定,采用气相色谱- 质谱联用技术鉴定其中的黄酮类化合物成分。结果:最佳提取工艺条件为乙醇体积分数95%、料液比1:9(g/mL)、提取时间48h、提取温度50℃,在此工艺条件下总黄酮含量为10.82%;并鉴定出了5 种黄酮类化合物。结论:该提取工艺和鉴定方法合理、简单,可作为提取与开发新疆产蜂胶总黄酮的方法和指标之一。

关键词: 蜂胶, 总黄酮, 提取工艺, 分光光度法, 气相色谱- 质谱联用法

Abstract:

Objective: The optimal process for total flavonoids extraction from Xinjiang propolis was determined, and the flavonoid composition of Xinjiang propolis was analyzed. Methods: Combined single factor and orthogonal array design methods were used to optimize the process conditions for total flavonoids extraction. The quantitative analysis of flavonoids was performed spectrometrically at 502 nm using rutin as a reference substance. Flavonoid composition analysis was achieved by using GC-MS. Results: The optimal extraction conditions were using a 9-fold volume of 95% ethanol as an extraction solvent for the 48 h extraction of the raw material at 50 ℃. Under these conditions, the extraction efficiency of total flavonoids was 10.82%. Conclusion: The extraction process and the analytical method are both reliable and simple, which can provide useful approaches for the extraction and exploitation of total flavonoids from Xinjiang propolis.

Key words: propolis, total flavonoids, extraction process, spectrophotometry, gas chromatography-mass spectrometry

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