食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 240-243.doi: 10.7506/spkx1002-6630-201102056

• 分析检测 • 上一篇    下一篇

超临界CO2 萃取鸡腿菇中的挥发性风味成分

李双石1,2,兰蓉1,*,张晓辉1,马越1,陈亮3   

  1. 1.北京电子科技职业学院,北京      100029;2.中国农业大学食品科学与营养工程学院,北京      100083;
    3.北京化工大学生命科学与技术学院,北京      100029
  • 收稿日期:2010-04-02 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 兰蓉 E-mail:lanrong1974@126.com
  • 基金资助:

    北京市教委科研基地项目;北京市属高等学校人才强教深化计划项目;北京电子科技职业学院重点科技类课题(YZK1009008)

Supercritical Carbon Dioxide Fluid Extraction and GC-MS Analysis of Volatile Flavor Compounds in Coprinus comatua Fruiting Body

LI Shuang-shi,LAN Rong,ZHANG Xiao-hui,MA Yue,CHEN Liang   

  1. 1. Beijing Electronic Science and Technology Vocational College, Beijing      100029, China;
    2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing      100083, China;
    3. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing      100029, China)  
  • Received:2010-04-02 Online:2011-01-25 Published:2011-01-10

摘要: 运用超临界CO2 萃取技术提取鸡腿菇中的挥发性风味成分,采用正交试验研究萃取温度、萃取压力、分离温度、分离压力等因素对鸡腿菇挥发性风味成分萃取率的影响。结果表明:最佳提取工艺条件为萃取温度55℃、萃取压力20MPa、分离温度25℃、分离压力8MPa。通过气相色谱- 质谱联用技术(GC-MS)分析萃取物,鉴定出25 种主要成分,其中亚油酸(52.67%)、硬脂酸(27.77%)和棕榈酸(13.66%)是构成鸡腿菇风味的主要挥发性成分。

关键词: 鸡腿菇, 挥发性风味成分, 超临界CO2 萃取, 气相色谱- 质谱法

Abstract: The extraction of volatile compounds from the fruiting body of Coprinus comatua was carried out using supercritical carbon dioxide fluid extraction technique, and the effects of the pressure and temperature for extraction and separation on extract yield were investigated by 9-run orthogonal array design experiments. The optimal temperature and pressure were 55 ℃ and 20 MPa for extraction and 25 ℃ and 8 MPa for separation. In the extract obtained under these conditions, 25 major components was identified by GC-MS, such as 9,12-octadecadienoic acid (52.67%), octadecenoic acid (27.77%) and hexadecenoic acid (13.66%).

Key words: Coprinus comatua, volatile flavor compounds, supercritical CO2 fluid extraction, gas chromatographymass spectrometry

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