食品科学 ›› 2011, Vol. 32 ›› Issue (1 ): 14-18.doi: 10.7506/spkx1002-6630-201101004

• 基础研究 • 上一篇    下一篇

不同防腐剂对3 种模式腐败菌抑菌效果的比较

陈南南1,徐 歆1,商丰才1,黄 琴1,陈有亮2,李卫芬1 ,*   

  1. 1. 浙江大学动物科学学院饲料科学研究所,教育部动物分子营养学重点实验室 2.浙江大学动物科学学院动物产品加工实验室
  • 收稿日期:2010-03-22 修回日期:2010-12-07 出版日期:2011-01-15 发布日期:2010-12-28
  • 通讯作者: 李卫芬 E-mail:wfli@zju.edu.cn
  • 基金资助:

    浙江省重大科研专项(2006C12066)

Comparative Study on Bacteriostasis of Different Preservatives for Three Typical Spoilage Bacteria

CHEN Nan-nan1,XU Xin1,SHANG Feng-cai1,HUANG Qin1,CHEN You-liang2,LI Wei-fen1,*   

  1. 1. Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Institute of Feed Science, College of Animal Science,
    Zhejiang University, Hangzhou 310029, China;2. Laboratory of Animal Product Processing, College of Animal Science,
    Zhejiang University, Hangzhou 310029, China
  • Received:2010-03-22 Revised:2010-12-07 Online:2011-01-15 Published:2010-12-28
  • Contact: LI Wei-fen E-mail:wfli@zju.edu.cn

摘要:

目的:研究食品防腐剂对腐败菌的抑菌效果。方法:采用微量稀释法测定不同防腐剂对藤黄微球菌、枯草芽孢杆菌和大肠杆菌的最小抑菌质量浓度(MIC)。结果:对于藤黄微球菌(G+),抑菌效果最好的是乳酸链球菌素(Nisin)与ε- 聚赖氨酸,MIC 均为12.5μg/mL;对于枯草芽孢杆菌(G+),抑菌效果最好的是丙酸钙、丁二酮、柠檬酸、柠檬醛和D- 异抗坏血酸钠,MIC 均为6.25μg/mL;对于大肠杆菌(G -),抑菌效果最好的是过氧化氢和丁二酮,MIC 均为25μg/mL。结论:不同防腐剂对3 种模式腐败菌的抑制效果有所差异,应复配使用。

关键词: 防腐剂, 腐败菌, 最小抑菌浓度, 抑菌作用

Abstract:

Objective: The inhibitory effects of twenty-three food preservatives on three typical spoilage bacteria were evaluated and compared. Methods: The minimal inhibitory concentrations (MIC) of different preservatives for Micrococcus luteus, Bacillus subtilis and E.coli were determined by micro-dilution assay. Results: Among the 23 tested food preservatives, Nisin and ε-poly-L-lysine had the best inhibitory effect on Micrococcus luteus (G+ cocci), and their MICs were both 12.5 μg/mL. For Bacillus subtilis (G+ bacillus), the best antimicrobials were calcium propionate, diacetyl, citric acid, citral and D-sodium isoascorbiate, presenting an identical MIC of 6.25μg/mL. As for E.coli (G- bacillus), hydrogen peroxide and diacetyl were the best preservatives, with an identical MIC of 25μg/mL. Conclusion: The bacteriostasis of various tested preservatives for the three typical spoilage bacteria is irregular and their combined use seems to be an approach for more effective bacteriostasis.

Key words: preservative, spoilage bacteria, minimal inhibition concentration, bacteriostasis

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