食品科学 ›› 2011, Vol. 32 ›› Issue (1 ): 115-119.doi: 10.7506/spkx1002-6630-201101028

• 生物工程 • 上一篇    下一篇

嗜热侧孢霉液体发酵产聚半乳糖醛酸酶条件优化

张 辉,桑 青,刘进森,孙占斌   

  1. 聊城大学生命科学学院
  • 收稿日期:2010-01-29 修回日期:2010-12-06 出版日期:2011-01-15 发布日期:2010-12-28
  • 通讯作者: 张辉 E-mail:zhanghui2005@lcu.edu.cn
  • 基金资助:

    山东省科技厅优秀中青年科学家奖励基金项目(2005gg3202084)

Optimization of Culture Medium and Liquid-state Fermentation Conditions for Polygalacturonase Production by Thermophilic sporotrichum

ZHANG Hui,SANG Qing,LIU Jin-sen,SUN Zhan-bin   

  1. (School of Life Sciences, Liaocheng University, Liaocheng 252059, China )
  • Received:2010-01-29 Revised:2010-12-06 Online:2011-01-15 Published:2010-12-28
  • Contact: ZHANG Hui E-mail:zhanghui2005@lcu.edu.cn

摘要:

对嗜热侧孢霉(Thermophilic sporotrichum)液体发酵产聚半乳糖醛酸酶条件进行研究。得出最佳产酶培养基条件为:麸皮1.5%、(NH4)2SO4 0.5%、苹果皮粉3%、KH2PO4 0.1%、MgSO4·7H2O 0.025%,pH6.0;发酵条件为:装液量45mL/250mL 三角瓶,培养温度50℃,转速170r/min,培养时间96h。最佳条件下培养,酶比活力为2768.97U/mL。

关键词: 聚半乳糖醛酸酶, 嗜热侧孢霉, 液体发酵

Abstract:

Thermophilic sporotrichum was cultured in a liquid medium with shaking for polygalacturonase production in this study. To improve polygalacturonase production, the culture medium composition and four fermentation conditions were optimized. The optimal culture medium composition found was composed of wheat bran 1.5%, (NH4)2SO4 0.5%, apple peel powder 3%, KH2PO4 0.1% and MgSO4·7H2O 0.025%, pH 6.0. The optimal values of the volume of the medium in a 250 mL conical flask, fermentation temperature and duration and shaking speed were determined to be 45 mL, 50 ℃, 96 h and 170 r/min, respectively. The fermentation broth obtained under the optimized conditions presented a polygalacturonase activity of 2768.97 U/mL.

Key words: polygalacturonase, Thermophilic sporotrichum, liquid-state fermentation

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