食品科学 ›› 2011, Vol. 32 ›› Issue (1 ): 236-239.doi: 10.7506/spkx1002-6630-201101057

• 营养卫生 • 上一篇    下一篇

体外消化/Caco-2 细胞模型评价面粉锌生物利用率

雷激1,张明秋2,张勇3,黄承钰2,*,白琳2   

  1. 1. 西华大学生物工程学院 2.四川大学华西公共卫生学院 3.中国农业科学院作物科学研究所
  • 收稿日期:2010-02-23 出版日期:2011-01-15 发布日期:2010-12-28
  • 通讯作者: 黄承钰 E-mail:hcynuph@163.com
  • 基金资助:

    国际合作Harvestplus-China 挑战项目(#8231);西华大学人才基金项目(R0910507);食品生物技术四川省高校重点实验室项目(川教2006-313)

Assessment of Zn Bioavailability in Wheat Flours Using an in vitro Digestion/Caco-2 Cell Model

LEI Ji1,ZHANG Ming-qiu2,ZHANG Yong3,HUANG Cheng-yu2,*,BAI Lin2   

  1. 1. School of Bioengineering, Xihua University, Chengdu 610039, China ;
    2. West China School of Public Health, Sichuan University, Chengdu 610041, China;
    3. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-02-23 Online:2011-01-15 Published:2010-12-28
  • Contact: HUANG Cheng-yu E-mail:hcynuph@163.com

摘要:

采用体外消化/Caco-2 细胞模型对10 种小麦粉进行锌生物利用率比较。以锌吸收率(Caco-2 细胞中的锌含量占加入到细胞的面粉消化液总锌量比例)反映锌生物利用率(Zn bioavailability,Zn BV)。发现不同面粉间锌生物利用率差异具有统计学意义,出粉率78% 的安阳中优9507 是面粉锌吸收率和可吸收锌量都高的品种,它将进入下一步人体实验进行营养功效的验证。

关键词: 小麦, 面粉, 体外消化/Caco-2 细胞模型, 锌, 生物利用率

Abstract:

Zinc bioavailability in 10 wheat flour varieties was assessed by using an in vitro/Caco-2 cell culture model to provide scientific foundation for further human trials and reach nutritional purpose in wheat breeding. Zn bioavailability (Zn BV) was defined as the percentage of the amount of zinc applied to Caco-2 cell monolayer and was consumed by the cells. Significant differences were detected in Zn BV among 10 wheat flour varieties, among which Zhongyou 9507 from Anyang had the highest Zn BV, which would be further tested in human trials for nutritional function study.

Key words: wheat, wheat flour, in vitro/ Caco-2 cell culture model, zinc, bioavailability

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