食品科学 ›› 2010, Vol. 31 ›› Issue (24): 46-49.doi: 10.7506/spkx1002-6630-201024009

• 工艺技术 • 上一篇    下一篇

纤维素酶酶解醇葛根素研究

王星敏1,2,3,殷钟意1,3,李 鑫1,张渝文   

  1. 1. 重庆工商大学环境与生物工程学院 2. 重庆大学资源及环境科学学院 3.重庆工商大学废油资源化技术与装备教育部工程中心
  • 收稿日期:2010-09-21 修回日期:2010-11-13 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 王星敏 E-mail:wxm0826@ctbu.edu.cn
  • 基金资助:

    重庆市科技攻关项目(cstc,2010AC1061);重庆市教委科技项目(cqjwKJ100708)

Ethanol Extraction of Puerarin by Cellulase Hydrolysis

WANG Xing-min1,2,3,YIN Zhong-yi1,3,LI Xin1,ZHANG Yu-wen1   

  1. 1. School of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067,
    China;2. School of Resources and Environmental Science; Chongqing University, Chongqing 400033, China;3. Engineering
    Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education, Chongqing Technology and Business
    University, Chongqing 400067, China
  • Received:2010-09-21 Revised:2010-11-13 Online:2010-12-25 Published:2010-12-29
  • Contact: WANG Xing-min1 E-mail:wxm0826@ctbu.edu.cn

摘要:

以提取葛粉后的葛根废渣为研究对象,采用微生物和代谢物酶解提制葛根素,通过高效液相色谱法分析葛根素含量,考察影响葛根素溶出的酶解时间、酶量、pH 值、固液比等参数,探索微生物与底物葛渣间的生物交互作用。实验结果表明:葛根废渣5g,纤维素酶量为底物40%,葛根废渣与水的质量比为1:10,pH 值为5,在30℃酶解36h 后,经乙醇浸提3h,可提取葛根素33.54mg,为葛根废渣的资源化再利用提供实验基础。

关键词: 纤维素酶, 葛根素, 葛根废渣, 酶解, 交互作用

Abstract:

Puerarin was extracted by enzymatic hydrolysis with microbe and cellulase from waste pueraria. High performance liquid chromatography was used to determine puerarin content for exploring the effects of enzymatic hydrolysis time, enzyme addition amount, pH and material-liquid ratio as well as cross-interaction on extraction rate of puerarin. Results indicated that pueraria amount of 5 g, cellulose concentration of 40% compared with pueraria weight, pueraria-water ratio of 1:10, enzymatic hydrolysis pH of 5, enzymatic hydrolysis temperature of 30 ℃, enzymatic hydrolysis time of 36 h and ethanol extraction time of 3 h. Under the optimal enzymatic hydrolysis and extraction conditions, the yield of puerarin was up to 33.54 mg. These investigations will provide a theoretical reference for the exploration and utilization of pueraria.

Key words: cellulase, puerarin, pueraria waste, enzymolysis, biological interaction

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