食品科学 ›› 2010, Vol. 31 ›› Issue (24): 66-69.doi: 10.7506/spkx1002-6630-201024013

• 工艺技术 • 上一篇    下一篇

向日葵盘总黄酮提取工艺优化及含量测定

杨树平1,韩立军2,陈晓云1,许瑞波1,蒋迎道1   

  1. 1.淮海工学院化学工程学院 2.淮海工学院理学院
  • 收稿日期:2010-09-21 修回日期:2010-11-10 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 杨树平 E-mail:spyang69320@yahoo.cn
  • 基金资助:

    江苏省海洋资源开发研究院科技开放基金课题(JSIMR09D02);淮海工学院自然科学基金项目(KX10019);
    淮海工学院引进人才启动基金项目(KK01064)

Extraction and Determination of Total Flavonoids from Helianthus annuus L.

YANG Shu-ping1,HAN Li-jun2,CHEN Xiao-yun1,XU Rui-bo1,JIANG Ying-dao1   

  1. 1. School of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China;
    2. School of Mathematics and Science, Huaihai Institute of Technology, Lianyungang 222005, China
  • Received:2010-09-21 Revised:2010-11-10 Online:2010-12-25 Published:2010-12-29
  • Contact: YANG Shu-ping E-mail:spyang69320@yahoo.cn

摘要:

为研究向日葵盘总黄酮提取最优条件,采用正交试验L9(33)考察乙醇体积分数、提取温度和提取时间对向日葵盘中总黄酮提取率的影响。用紫外分光光度法测定提取液中总黄酮含量,以总黄酮含量作为提取工艺优化指标,得出向日葵盘中总黄酮的最佳提取工艺条件为A1B3C3,即乙醇体积分数50%、提取温度80℃、反应时间2h,提取效果最好,向日葵盘中总黄酮含量高达33.6436mg/g。

关键词: 向日葵盘, 总黄酮, 工艺优化, 提取

Abstract:

In order to investigate the optimal extraction processing conditions of total flavonoids from Helianthus annuus L, orthogonal experiments were used to explore the effects of ethanol concentration, extraction temperature and extraction time on extraction rate of total flavonoids. The content of total flavonoids was determined by ultraviolet (UV) spectrometry method. Results indicated that the optimal extraction processing conditions were ethanol concentration of 50%, extraction temperature of 80 ℃and extraction duration of 2 h. The maximum extraction rate of 33.6436 mg/g was achieved under the optimal extraction conditions.

Key words: Helianthus annuus L., total flavonoids, optimization, extraction

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