食品科学 ›› 2010, Vol. 31 ›› Issue (24): 90-94.doi: 10.7506/spkx1002-6630-201024019

• 工艺技术 • 上一篇    下一篇

响应面法优化微波提取野菊花抗氧化物质

褚福红1,2,陆 宁1,于 新2,*,蒋 雨3   

  1. 1.安徽农业大学茶与食品科技学院 2. 仲恺农业工程学院轻工食品学院 3.四川农业大学食品学院
  • 收稿日期:2010-02-03 修回日期:2010-08-30 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 于新 E-mail:yuxin1959@yahoo.com.cn
  • 基金资助:

    广东省科技计划项目(2005B20401002)

Optimization of Microwave Extraction Conditions for Antioxidant Materials from Chrysanthemum indicum L. by Response Surface Methodology

CHU Fu-hong1,2,LU Ning1,YU Xin2,*,JIANG Yu3   

  1. 1. College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    2. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    3. Food Science College, Sichuan Agricultural University, Ya an 625014, China
  • Received:2010-02-03 Revised:2010-08-30 Online:2010-12-25 Published:2010-12-29
  • Contact: YU Xin E-mail:yuxin1959@yahoo.com.cn

摘要:

以野菊花花蕾为材料,研究其提取物的抗氧化活性。在乙醇体积分数、提取时间、料液比等单因素试验的基础上,根据中心组合试验设计原理,应用响应面分析法研究各因素的显著性和交互作用的强弱,对野菊花微波提取工艺进行优化。结果表明,微波提取野菊花清除DPPH 自由基物质的优化工艺条件为乙醇体积分数50%、料液比1:27(g/mL)、提取时间51s。提取物质量浓度为0.6mg/mL 时,DPPH 自由基实际清除率达到83.23%,与理论预测值83.10 % 的绝对误差为0.13%。

关键词: 响应面分析法, 野菊花, 微波提取, DPPH 自由基

Abstract:

Chrysanthemum indicum L. was used as the raw material to extract antioxidant materials by microwave technology. On the basis of single-factor experimental results and central composite design principle, the optimal extraction processing parameters were explored by response surface experiments through evaluating the effects of ethanol concentration, extraction time, material-liquid ratio and their cross-interactions on antioxidant activity of the extract. Results indicated that the optimal extraction processing parameters were ethanol concentration of 50% (V/V), microwave treatment time of 51 s and material-liquid ratio of 1:27 (g/mL). Under these optimal extraction conditions, the scavenging rate of the extract from Chrysanthemum indicum L. at the concentration of 0.6 mg/mL on DPPH free radicals was up to 83.10%, which was close to the predicted value of 83.23% with an absolute error of 0.13%.

Key words: response surface method (RSM), Chrysanthemum indicum L., microwave extraction, DPPH free radicals

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