花果山野生蕨菜多糖和黄酮的提取及含量测定

doi:10.7506/spkx1002-6630-201024026

食品科学 ›› 2010, Vol. 31 ›› Issue (24): 124-127.doi: 10.7506/spkx1002-6630-201024026

花果山野生蕨菜多糖和黄酮的提取及含量测定

夏海涛，刘玉芬，王蓉，李肖雅

淮海工学院化学工程学院

收稿日期:2010-08-06 修回日期:2010-11-04 出版日期:2010-12-25 发布日期:2010-12-29
通讯作者: 夏海涛 E-mail:xht161006@hhit.edu.cn

Extraction and Content Determination of Polysaccharides and Flavonoids from Wild Pteridium aquilinum

XIA Hai-tao，LIU Yu-fen，WANG Rong，LI Xiao-ya

School of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China

Received:2010-08-06 Revised:2010-11-04 Online:2010-12-25 Published:2010-12-29
Contact: XIA Hai-tao E-mail:xht161006@hhit.edu.cn

摘要/Abstract

摘要：

以连云港花果山野生蕨菜干粉为原料，通过正交试验选择最佳提取条件，用分光光度法测定总黄酮和多糖的含量。结果表明：蕨菜中多糖提取的最优条件为蕨菜与水料液比1:20(g/mL)、提取温度90℃、提取时间1.5h、提取次数2 次。新鲜蕨菜干粉中多糖含量为1.39%，三月枯蕨菜干粉中多糖含量为7.08%；提取蕨菜中黄酮类物质的最优条件为蕨菜与70% 乙醇比1:10(g/mL)、提取温度90℃、提取时间2h、提取次数2 次，测得新鲜蕨菜干粉中总黄酮含量为4.90%，三月枯蕨菜干粉中总黄酮含量为3.11%。

关键词: 多糖, 黄酮, 蕨菜

Abstract:

The optimal extraction processing of polysaccharides and total flavonoids from wild Pteridium aquilinum powder collected from Huaguo Mountain of Lianyungang were explored by orthogonal experiments. The contents of total flavonoids and polysaccharides were determined by spectrophotometry. The optimal extraction conditions for polysaccharides were materialliquid ratio of 1:20, extraction temperature of 90 ℃, extraction repeat number of 2 and extraction time of 1.5 h each time. Under the optimal extraction conditions, the contents of polysaccharides in fresh Pteridium aquilinum and dry Pteridium aquilinum were 1.39% and 7.08%, respectively. The optimal extraction conditions for total flavonoids were material-70% ethanol ratio of 1:10, extraction temperature of 90 ℃, extraction repeat number of 2 and extraction time of 2 h each time. Under the optimal extraction conditions, the contents of total flavonoids in fresh Pteridium aquilinum and dry Pteridium aquilinum were 4.90% and 3.11%, respectively.

Key words: polysaccharide, flavonoids, Pteridium aquilinum

中图分类号:

TS247

夏海涛，刘玉芬，王蓉，李肖雅. 花果山野生蕨菜多糖和黄酮的提取及含量测定[J]. 食品科学, 2010, 31(24): 124-127.

XIA Hai-tao，LIU Yu-fen，WANG Rong，LI Xiao-ya. Extraction and Content Determination of Polysaccharides and Flavonoids from Wild Pteridium aquilinum[J]. FOOD SCIENCE, 2010, 31(24): 124-127.

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花果山野生蕨菜多糖和黄酮的提取及含量测定

Extraction and Content Determination of Polysaccharides and Flavonoids from Wild Pteridium aquilinum

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