食品科学 ›› 2010, Vol. 31 ›› Issue (24): 255-258.doi: 10.7506/spkx1002-6630-201024054

• 分析检测 • 上一篇    下一篇

牛乳中氨苄青霉素残留的受体分析法测定

孙永海1,张 杰2,韩 勇3,李倬琳2,李铁柱2 ,*   

  1. 1.吉林大学生物与农业工程学院 2.吉林省农业科学院农产品加工研究中心 3.中国国际工程咨询公司
  • 收稿日期:2010-01-13 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 李铁柱 E-mail:ltzjlu@126.com
  • 基金资助:

    国家现代农业产业技术体系建设专项(nycytx-0502);国家公益性行业(农业)科研专项(200903043);
    中国博士后科学基金项目(20100471246);吉林省博士后科研项目(04006)

Determination of Ampicillin Residues in Milk by Receptor-binding Assay

SUN Yong-hai1,ZHANG Jie2,HAN Yong3,LI Zhuo-lin2,LI Tie-zhu2,*   

  1. 1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China;
    2. Center of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130124, China;
    3. China International Engineering Consulting Corporation, Beijing 100048, China
  • Received:2010-01-13 Online:2010-12-25 Published:2010-12-29
  • Contact: LI Tie-zhu E-mail:ltzjlu@126.com

摘要:

建立基于受体识别技术检测牛乳青霉素类抗生素残留的新方法。通过生物技术制备与青霉素类抗生素具有高度亲和力的受体蛋白质,进而以其为识别元件开发间接竞争氨苄青霉素残留检测方法。青霉素受体包被质量浓度5μg/mL,生物素化氨苄青霉素质量浓度500ng/mL 条件下,对其残留量进行测定,检出限为2μg/kg,有较好的精密度及回收率。在样品中残留的青霉素种类已知的前题下(以氨苄青霉素为例),本方法可以作为定量筛选方法。

关键词: 牛乳, 氨苄青霉素, 受体分析, 生物素, 亲和素

Abstract:

The receptor has been utilized to develop a novel microplate assay for the determination of ampicillin with intact beta-lactam structure in milk. The receptor with high affinity to penicillin is produced by biotechnology. Then the receptor is employed as an identification unit to develop an indirect competitive receptor-binding assay. The detection limit of ampicillin was 2 μg/kg in milk. The assay has been developed as screening test with the option for a quantitative assay, when the identity of the residual beta-lactam is known.

Key words: milk, ampicillin, receptor binding assays, biotin, avidin

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