食品科学 ›› 2010, Vol. 31 ›› Issue (24): 281-284.doi: 10.7506/spkx1002-6630-201024060

• 分析检测 • 上一篇    下一篇

高效液相法测定刺玫果中金丝桃苷的含量

钟方丽,王晓林* ,敬采月   

  1. 吉林化工学院化学与制药工程学院
  • 收稿日期:2010-02-22 修回日期:2010-10-22 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 王晓林 E-mail:wangxiaolin69@eyou.com
  • 基金资助:

    吉林省教育厅重点计划项目(吉教科合字[2006]第122 号)

Determination of Hyperin in Rosa davurica Pall. by HPLC

ZHONG Fang-li,WANG Xiao-lin*,JING Cai-yue   

  1. School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China
  • Received:2010-02-22 Revised:2010-10-22 Online:2010-12-25 Published:2010-12-29
  • Contact: WANG Xiao-lin E-mail:wangxiaolin69@eyou.com

摘要:

目的:建立刺玫果中金丝桃苷含量测定的方法。方法:采用高效液相色谱法测定,色谱柱为Shim-packVP-ODS C18(4.6mm × 150mm,5μm);以甲醇(A)和0.2% 磷酸溶液(B)进行梯度洗脱,流速为1.0mL/min;进样量20μL;检测波长360nm;柱温40℃。结果:金丝桃苷进样质量浓度在4.76~76.16μg/mL 范围内呈良好的线性关系,r=0.9993。平均回收率为98.06%,RSD 为1.72%。结论:采用高效液相色谱法测定刺玫果中金丝桃苷的含量,方法简便、准确,可以用于刺玫果的质量控制。

关键词: 刺玫果, 金丝桃苷, 高效液相色谱法

Abstract:

Objective: To establish a determination method for hyperin in Rosa davurica Pall.. Methods: A HPLC method was developed using Shim-pack VP-ODS C18 (4.6 mm × 150 mm, 5 μm) column as the stationary phase and CH3OH-0.2% H3PO4 as the mobile phase. The flow rate of mobile phase, injection volume of the sample and the column temperature were 1.0 mL/min, 20 μL and 40 ℃, respectively. The UV detection wavelength was 360 nm. Results: A good linear relationship between integral value of peak area and hyperin concentration in the range of 4.76-76.16 μg/mL (r = 0.9993) was observed. The average recovery rate of hyperin was 98.06% with the RSD of 1.72%. Conclusion: The HPLC determination of hyperin in Rosa davurica Pall. was characteristics of convenience and accuracy, which can be used for the quality control of Rosa davurica Pall.

Key words: Rosa davurica Pall., hyperin, HPLC method

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