食品科学 ›› 2010, Vol. 31 ›› Issue (24): 285-288.doi: 10.7506/spkx1002-6630-201024061

• 分析检测 • 上一篇    下一篇

基质固相分散术-UPLC 法检测禽蛋中对位红和苏丹红

侯晓林1,孙英健1,吴国娟1,苗 虹2,吴永宁2   

  1. 1.北京农学院动物科技系 2.中国疾病预防控制中心营养与食品安全所
  • 收稿日期:2010-01-08 修回日期:2010-10-25 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 侯晓林 E-mail:hxlsx@163.com
  • 基金资助:

    北京市属高等学校人才强教计划项目(PXM2009_014207_077729);国家“863”计划项目(2007AA06Z404)

Use of Matrix Solid-Phase Dispersion and Ultra Performance Liquid Chromatography for Determination of Para Red and SudanⅠ-Ⅳ in Egg Yolk

HOU Xiao-lin1,SUN Ying-jian1,WU Guo-juan1,MIAO Hong2,WU Yong-ning2   

  1. 1. Department of Animal Science, Beijing University of Agriculture, Beijing 102206, China;
    2. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China
  • Received:2010-01-08 Revised:2010-10-25 Online:2010-12-25 Published:2010-12-29
  • Contact: HOU Xiao-lin E-mail:hxlsx@163.com

摘要:

建立禽蛋中对位红和苏丹红Ⅰ~Ⅳ号5 种色素的液相色谱检测方法。采用基质固相分散术,以氧化铝作基质分散基体,混合均匀的样品用正己烷- 丙酮(99.5:0.5,V/V)直接洗脱,吹干后定容测定,外标法定量。5 种色素的平均回收率为63.8%~96.2%,平均变异系数为0.52%~10.07%,检测限为0.01~0.02mg/kg。在检测的100个样品中,1 例检出苏丹红Ⅳ号。

关键词: 禽蛋, 对位红, 苏丹红, 高效液相色谱, 基质固相分散

Abstract:

An ultra-performance liquid chromatography (UPLC) method combined with matrix solid-phase dispersion (MSPD) was established for determination of para red and sudanⅠ, Ⅱ, Ⅲ and Ⅳ in egg yolk. A total of 0.5 g egg yolk was mixed with 2 g of neutral alumina, then rinsed with 35 mL of hexane-acetone (0.5:99.5, V/V) through a 5 mL syringe. The eluent was heated to about 45℃ with nitrogen blowing to dryness and redissolved for UPLC analysis. Mean recovery for the five dyes ranged from 63.8% to 96.2%, with coefficient variation at 0.52%-10.07%. One sample was found containing sudan Ⅳ among the 100 samples.

Key words: egg yolk, para red, sudan Ⅰ-Ⅳ, ltra-performance liquid chromatography, maxtrix solid-phase dispersion

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