食品科学 ›› 2010, Vol. 31 ›› Issue (24): 305-307.doi: 10.7506/spkx1002-6630-201024065

• 分析检测 • 上一篇    下一篇

高效液相色谱法对绿茶中茶多酚含量的测定

侯冬岩,回瑞华,李铁纯,刁全平,魏 威,刘晓媛   

  1. 鞍山师范学院化学系
  • 收稿日期:2010-06-11 修回日期:2010-10-29 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 侯冬岩 E-mail:houdongyan2010@163.com
  • 基金资助:

    辽宁省教育厅科学技术基金项目 (20331079)

HPLC Determination of Polyphenols in Green Tea

HOU Dong-yan,HUI Rui-hua,LI Tie-chun,DIAO Quan-ping,WEI Wei,LIU Xiao-yuan   

  1. Department of Chemistry, Anshan Normal University, Anshan 114007, China
  • Received:2010-06-11 Revised:2010-10-29 Online:2010-12-25 Published:2010-12-29
  • Contact: HOU Dong-yan E-mail:houdongyan2010@163.com

摘要:

采用高效液相色谱法测定绿茶中茶多酚的含量。在色谱柱为Kromasil C18、流动相为乙酸:甲醇:N,N- 二甲基甲酰胺:水=1:1:40:58(V/V) 、色谱柱温30℃、进样量10μL、检测器为紫外检测器、检测波长280.0nm 的色谱条件下对绿茶中茶多酚含量进行测定。结果表明:回归方程为Y=1 × 10-7X - 0.0036(r=0.9995),方法变异系数小于3.04%,回收率为99.16%~100.60%;6 种绿茶样品茶多酚含量为62.07~110.86mg/g,说明产地不同,绿茶中茶多酚的含量相差较大。本方法简便、准确,适合绿茶中茶多酚含量的分析。

关键词: 绿茶, 高效液相色谱法, 茶多酚, 测定

Abstract:

The polyphenol contents in green tea were determined by high performance liquid chromatography method, using the mobile phase consisting of acetic acid, methyl alcohol, N,N- dimethylformamide and warter (1:1:40:58, V/V). The column temperature was 30 ℃, and the detection wavelength was 280.0 nm. The regression equation obtained was Y=1×10-7X-0.0036 (r =0.9995). The coefficient of variation was less than 3.04%, and the recovery was 99.16%-100.60%. The result indicates that the proposed method is simple , accurate and feasible for determination of polyphenols in the green teas.

Key words: green tea, HPLC, polyphenols, determination

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