食品科学 ›› 2010, Vol. 31 ›› Issue (24): 314-317.doi: 10.7506/spkx1002-6630-201024068

• 分析检测 • 上一篇    下一篇

碘量法测定发酵液中2-酮基-D-葡萄糖酸

冯晓燕1,周延政2,孙文敬1 , 2,王大明1,余泗莲3,刘敬泽1 ,*   

  1. 1. 河北师范大学生命科学学院
    2.江苏大学食品与生物工程学院
    3. 百勤异VC 钠有限公司
  • 收稿日期:2010-08-30 修回日期:2010-11-02 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 刘敬泽1 E-mail:liujingze@mail.hebtu.edu.cn
  • 基金资助:

    江苏大学科研启动基金项目(08JDG029);江西省主要学科学术和技术带头人培养计划项目(2008DD00600);
    江西省科技支撑计划项目(赣财教[2008]147号)

Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth

FENG Xiao-yan1,ZHOU Yan-zheng2,SUN Wen-jing1,2,WANG Da-ming1,YU Si-lian3,LIU Jing-ze1,*   

  1. 1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;2. School of Food and Biological Engineering,
    Jiangsu University, Zhenjiang 212013, China;3. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, Chi
  • Received:2010-08-30 Revised:2010-11-02 Online:2010-12-25 Published:2010-12-29
  • Contact: LIU Jing-ze1 E-mail:liujingze@mail.hebtu.edu.cn

摘要:

在优化样品处理条件的基础上,考察葡萄糖对2-酮基-D-葡萄糖酸测定的影响,改进发酵液中2-酮基-D-葡萄糖酸的碘量法测定技术。结果表明,滴定样品溶液所消耗的I2标准溶液的体积、样品溶液中2-酮基-D-葡萄糖酸质量浓度和葡萄糖质量浓度之间存在良好的线性关系,R2>0.9999;该方法精密度、稳定性和重现性良好,2-酮基-D-葡萄糖酸的平均回收率为95.68%,RSD为1.35%(n=5)。该方法准确、快速,操作简便,可用于发酵液中2-酮基-D-葡萄糖酸的定量检测。

关键词: 发酵液, 碘量法, 测定, 2-酮基-D-葡萄糖酸, 葡萄糖

Abstract:

Iodometric method was improved to prevent the interference of glucose in determination of 2-keto-D-gluconic acid  (2KGA) in fermentation broth. The results revealed a good linear relationship among the volumes of I2 standard solution consumed and concentrations of 2-keto-D-gluconic acid and glucose (R2>0.9999). The average recovery was 95.68% with the relative standard deviation (RSD) of 1.35% (n=5). The method developed here had good accuracy, stability and reproducibility, and was easily operated, hereby could be used for the determination of concentrations of 2-keto-D-gluconic acid in fermentation broth.

Key words: 2-keto-D-gluconic acid, glucose, fermentation broth, iodometry

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