两种羊肚菌胞内多糖体外抗氧化性

doi:10.7506/spkx1002-6630-201023018

食品科学 ›› 2010, Vol. 31 ›› Issue (23): 76-78.doi: 10.7506/spkx1002-6630-201023018

两种羊肚菌胞内多糖体外抗氧化性

杨芳^1,2，王新风^1,2,*，翁良¹，戈群妹¹，陈珊珊¹

1.淮阴师范学院生命科学学院资源微生物研究所
2.江苏省生态农业生物技术重点实验室

收稿日期:2010-09-14 出版日期:2010-12-15 发布日期:2010-12-29
通讯作者: 王新风 E-mail:wangxf@hytc.edu.cn
基金资助:
江苏省教育厅高新产业项目(JHZD04-006)；江苏省大学生实践创新训练项目(JSDXS20090212)；
淮阴师范学院教授基金项目(08HSJSK05)

Antioxidant Activity of Intracellular Polysaccharides (IPS) from Morchella esculenta L.

YANG Fang1,2，WANG Xin-feng1,2,*，WENG Liang1，GE Qun-mei1，CHEN Shan-shan1

1.Institute of Resource Microorganism, College of Life Sciences, Huaiyin Normal University, Huai an 223300, China；
2. Jiangsu Key Laboratory for Eco-Agricultural Biotechology around Hongze Lake, Huai an 223300, China

Received:2010-09-14 Online:2010-12-15 Published:2010-12-29
Contact: WANG Xin-feng E-mail:wangxf@hytc.edu.cn

摘要/Abstract

摘要：

用水提醇沉法提取北京羊肚菌(MB)和淮阴羊肚菌(MH)菌丝体胞内多糖(IPS1)，经脱色及脱蛋白得纯化多糖(IPS2)。采用水杨酸法测定IPS1 和IPS2 对·OH 的清除作用；邻苯三酚自氧化法测定IPS1 和IPS2 对O2·的抑制作用。结果表明： IPS1 和IPS2 均能明显地清除·OH 和抑制O2·的能力，在1～5mg/mL 质量浓度范围，抗氧化活性随质量浓度的增加而增加。MB 和MH 提取的IPS1 和IPS2 清除O2·能力差异不显著，MB 和MH 提取的IPS1清除·OH 能力的差异也不显著，MH 提取的IPS1 和IPS2 之间清除·OH 能力差异显著，质量浓度为5mg/mL 时，IPS1 清除能力只有22.57%，而IPS2 清除能力高达46.27%，表明MH 的胞内多糖的清除·OH 能力明显高于MB 胞内多糖。

关键词: 羊肚菌, 胞内多糖, 抗氧化性

Abstract:

The crude intracellular polysaccharides (CIPSMB and CIPSMH) extracted from the mycelia of Morchella esculenta L. from Beijing (MB) and Huaiyin (MH) areas by water extraction and alcohol precipitation were subjected to decolorization and deproteinization to obtain purified CIPSMB and CIPSMH, denoted as PIPSMB and PIPSMH, respectively. The scavenging effects of the four samples on hydroxyl and superoxide anion free radicals were tested by salicylic acid method and pyrogallol autoxidation method, respectively. It was observed that all the four samples were able to obviously scavenge both free radicals, displaying a positive concentration-dependent response in a concentration range between 1 mg/mL and 5 mg/mL. No statistical significance in the ability to scavenge superoxide anion free radicals was found between CIPSMB and PIPSMB and between CIPSMH and PIPSMH. CIPSMB and CIPSMH had no significant difference in the ability to scavenge hydroxyl free radicals. However, there was a significant difference in the ability to scavenge hydroxyl free radicals observed between CIPSMH and PIPSMH; the scavenging rate of CIPSMH at a concentration of 5 mg/mL was only 22.57%, whereas that of PIPSMH was as high as 46.27%, indicating that purification greatly enhances the hydroxyl free radical scavenging capacity of intracellular polysaccharides from the mycelia of Morchella esculenta L. from Huaiyin area.

Key words: Morchella esculenta L., intracellular polysaccharide, antioxidant activity

中图分类号:

TS201.3

杨芳1,2，王新风1,2,*，翁良1，戈群妹1，陈珊珊1. 两种羊肚菌胞内多糖体外抗氧化性[J]. 食品科学, 2010, 31(23): 76-78.

YANG Fang1,2，WANG Xin-feng1,2,*，WENG Liang1，GE Qun-mei1，CHEN Shan-shan1. Antioxidant Activity of Intracellular Polysaccharides (IPS) from Morchella esculenta L.[J]. FOOD SCIENCE, 2010, 31(23): 76-78.

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两种羊肚菌胞内多糖体外抗氧化性

Antioxidant Activity of Intracellular Polysaccharides (IPS) from Morchella esculenta L.

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