食品科学 ›› 2010, Vol. 31 ›› Issue (23): 96-100.doi: 10.7506/spkx1002-6630-201023023

• 基础研究 • 上一篇    下一篇

冬夏鲤鱼鱼皮和鱼骨胶原蛋白稳定性比较

段 蕊1,2,*,张俊杰1,2,陈 玲1,今野久仁彦3,许 冰1   

  1. 1.淮海工学院海洋学院 2.江苏省海洋资源开发研究院 3.日本北海道大学水产科学研究院
  • 收稿日期:2010-03-02 修回日期:2010-11-13 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 段蕊 E-mail:duanrui526@yahoo.com.cn
  • 基金资助:

    连云港市科技攻关计划(工业)项目(CG0803-3);江苏省海洋资源开发研究院科技开放基金项目(JSIMR09D04)

Comparison on the Stability of Collagens from Skin and Bone of Carp (Cyprinus carpio) Caught in Winter and Summer

DUAN Rui1,2,*,ZHANG Jun-jie1,2,CHEN Ling1,KONNO Kunihiko3,XU Bing1   

  1. 1. School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China;
    2. Jiangsu Marine Resources Development Research Institute, Lianyungang 222005, China;
    3. Faculty of Fisheries Sciences, Hokkaido University, Hokkaido 041-8611, Japan
  • Received:2010-03-02 Revised:2010-11-13 Online:2010-12-15 Published:2010-12-29
  • Contact: DUAN Rui E-mail:duanrui526@yahoo.com.cn

摘要:

从冬季(2008 年2 月)和夏季(2008 年7 月)的鲤鱼鱼皮和鱼骨中分别提取酸溶性胶原蛋白,在一定温度下加热,通过蛋白酶的酶解和圆二色谱(CD)法研究其热稳定性。结果显示:冬季鱼皮和鱼骨酸溶性胶原蛋白的变性温度分别为31.0℃和 32.8℃,比夏季鱼皮和鱼骨酸溶性胶原蛋白的变性温度分别低1.0℃和0.6℃。通过圆二色谱(CD)法得到了与此一致的结果。测定结果显示,来源于夏季鱼皮和鱼骨胶原蛋白的热稳定性要优于冬季鱼皮和鱼骨胶原蛋白。

关键词: 胶原蛋白, 热稳定性, 胃蛋白酶酶解, 圆二色谱

Abstract:

The acid-soluble collagens (ASC) were isolated from skin and bone of carp (Cyprinus carpio) caught in the winter (February in 2008) and summer (July in 2008). Thermal stability of collagens was studied by the increased pepsin digestibility upon heating and by circular dichroism (CD) spectroscopy. Collagens from skin and bone in the winter (w-skin ASC and w-bone ASC) exhibited transition curves with midpoints of 31.0 ℃and 32.8 ℃, respectively, which were 1.0 ℃ and 0.6 ℃ below their equivalents from summer season fish. The CD spectrum changes of collagens upon heating were in accordance with the results of pepsin digestion. The results indicated that collagens from skin and bone of summer carp were more stable than their equivalents of winter.

Key words: collagen, thermostability, pepsin digestion, circular dichroism

中图分类号: