关键词: 单核细胞增多性李斯特菌, p60 蛋白, 表达条件, 优化

Abstract:

In this study, the optimal conditions for the expression of Listeria monocytogenes p60 protein, for which the recombinant expression vector pET-28a-p60 and the recombinant E. coli BL21(DE3) host strain were available, were investigated. The results showed that the optimal expression process determined was that the host strain was enriched to a OD600nm value between 0.7 and 0.8, and IPTG was then added to a final concentration of 1 mmol/L, followed by 6 h expression on a shake table with a shaking speed of 150 r/min at 25 ℃, and that after the expression, the content of p60 protein as a percentage of total protein content was 42.32%, and soluble protein occupied around 85.36% of the total p60 protein.

Key words: Listeria monocytogenes, p60 protein, expression condition, optimization