食品科学 ›› 2010, Vol. 31 ›› Issue (23): 174-177.doi: 10.7506/spkx1002-6630-201023040

• 生物工程 • 上一篇    下一篇

Lactobacillus paracaseiW2合成苯乳酸培养条件的优化

刘 戈1,王立梅2,3,*   

  1. 1.吉林农业大学食品工程学院 2.常熟理工学院生物与食品工程系 3.苏州市食品生物技术重点实验室
  • 收稿日期:2010-09-16 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 王立梅 E-mail:wlmqb@126.com
  • 基金资助:

    江苏省科学技术局(BE2008391);常熟市科技局资助项目(CN200921)

Optimization of Culture Medium for Phenyllactic Acid Production by Lactobacillus paracasei W2

LIU Ge1,WANG Li-mei2,3,*   

  1. 1. College of Food Engineering, Jilin Agricultural University, Changchun 130118, China;2. Department of Biotechnology and
    Food Engineering, Changshu Institute of Technology, Changshu 215500, China ;3. Suzhou Key Laboratory of Food and
    Biotechnology, Changshu 215500, China
  • Received:2010-09-16 Online:2010-12-15 Published:2010-12-29
  • Contact: WANG Li-mei E-mail:wlmqb@126.com

摘要:

对苯乳酸(PLA)高产菌株副干酪乳杆菌Lactobacillus paracaseiW2 的培养条件进行优化,以提高PLA 合成能力。采用Plackett-Burman(PB)试验设计法,筛选出3 个主要影响因子:葡萄糖、吐温-80、苯基丙酮酸(PPA)。通过最陡爬坡法和响应面分析方法(RSM)确定葡萄糖质量浓度19.5g/L、吐温-80 4mL/L、苯基丙酮酸质量浓度3g/L。在此优化条件下,Lactobacillus paracaseiW2 发酵合成苯乳酸的产量达到801mg/L,比优化前提高1.4 倍。

关键词: Lactobacillus paracaseiW2, 苯乳酸, 最陡爬坡法, Plackett- Burman 设计, 响应面分析

Abstract:

The optimal medium for phenyllactic acid (PLA) production by Lactobacillus paracasei W2 was investigated in order to improve PLA yield. Three most significant components affecting PLA production were selected out of seven components using Plackett-Burman design, namely glucose, Tween-80 and phenylpyruvic acid. Their optimal concentrations were determined by steepest ascent method and response surface methodology (RSM) to be 19.5 g/L, 4 mL/L and 3 g/L, respectively. PLA production reached 801 mg/L under these conditions, 1.4-fold higher than before optimization.

Key words: Lactobacillus paracasei W2, PLA, steepest ascent method, Plackett-Burman design, response surface methodology

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